Phosphoric acid acs reagent 85

Ethyl acetate and ethanol were purchased from Acros Organics (Pittsburgh, PA) and used as received, and HPLC grade methanol and acetonitrile were purchased from Fisher Scientific (Pittsburgh, PA). Trifluoroacetic acid spectrophometric grade 99% was purchased from Aldrich Company (Milwaukee, WI) and phosphoric acid ACS reagent 85% was purchased from Sigma Chemical Company (St. Louis, MO). Water used in this study was filtered through a double deionized purification system (Milli Q Plus Water System) from Millipore Co. (Bedford, MA).
FeSSIF and acetate buffer were prepared using sodium taurocholate (NaTC) purchased from Sigma Chemical Company (St. Louis, MO), lecithin purchased from Fisher Scientific (Pittsburgh, PA), sodium hydroxide pellets (NaOH) purchased from J.T. Baker (Philipsburg, NJ), and acetic acid and potassium chloride (KCl) purchased from Acros Organics (Pittsburgh, PA).

Ethyl acetate and ethanol were purchased from Acros Organics (Pittsburgh, PA) and used as received, and HPLC grade methanol and acetonitrile were purchased from Fisher Scientific (Pittsburgh, PA). Trifluoroacetic acid spectrophometric grade 99% was purchased from Aldrich Company (Milwaukee, WI) and phosphoric acid ACS reagent 85% was purchased from Sigma Chemical Company (St. Louis, MO). Water used in this study was filtered through a double deionized purification system (Milli Q Plus Water System) from Millipore Co. (Bedford, MA).
Tween 80 solutions, FeSSIF, and acetate buffer were prepared using Tween 80 purchased from Sigma Chemical Company (St. Louis, MO), sodium taurocholate (NaTC) purchased from Sigma Chemical Company (St. Louis, MO), lecithin purchased from Fisher Scientific (Pittsburgh, PA), sodium hydroxide pellets (NaOH) purchased from J.T. Baker (Philipsburg, NJ), and acetic acid and potassium chloride (KCl) purchased from Acros Organics (Pittsburgh, PA).