3 4 5 dimethylthoazol 2yl 2 5 diphenyl 2h tetrazolium bromide mtt

PT and docetaxel (internal standard, IS) were gifted by Korea United Pharm, Inc. (Seoul, Korea). Isopropyl palmitate, palmitic, myristic and stearic acid were purchased from Daejung Chemical (Cheongwon, Korea). Tween 80, Tween 20, GMS, oleic acid, Span 85 and 80, phosphoric acid and mannitol were obtained Samchun Chemical (Pyungtaek, Korea). Capmul^® MCM-NF and -EP were purchased from Abitec Corporation (Columbus, OH, USA). Gelucire 44/14, 33/01, 43/01, 50/13, Capryol™ PGMC, Compritol^® 888 ATO, Capryol 90, Labrafac™ WL 1349 and CC, Labrasol, Cremophor^® EL, Labrafil^® M 2125 CS, Peceol, Lauroglycol 90, Transcutol^® HP, Lauroglycol™ FCC, precirol ATO, and Labrafil^® M 1944 CS were obtained by Gattefossé (Saint Priest, Cedex, France). Poloxamer 188, 407 and Solutol^® HS-15 were obtained from BASF (Ludwigshafen, Germany). Dimethyl sulfoxide (DMSO), DAPI, 3-(4,5-dimethylthoazol-2yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) and rhodamine–phalloidin were obtained from Sigma-Aldrich (St. Louis, MO, USA). SK-OV-3 cells, an ovarian cancer cell line, were obtained from the Korean Cell Line Bank (Seoul, Korea). High-performance liquid chromatography (HPLC)-grade methanol (MeOH) and acetonitrile (ACN) were obtained from JT Baker (Phillipsburg, NJ, USA).

USL was provided from the Korea Institute of Oriental Medicine (Daejeon, Korea). Acetic acid, monosodium phosphate, disodium phosphate, sodium chloride, hydrochloric acid, benzalkonium chloride, and sodium hydroxide were purchased from Samchun Chemical (Pyungtaek, Korea). Ecdysterone as a reference substance, dimethyl sulfoxide (DMSO), 3-(4,5-dimethylthoazol-2yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT), and sodium CMC (average M_w ≒250,000, degree of substitution 0.7) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Dulbecco’s modified Eagle’s medium (DMEM), fetal bovine serum (FBS), penicillin-streptomycin, and trypsin- ethylenediaminetetraAcetic acid (EDTA) were purchased from Gibco BRL (Gaithersburg, MD, USA). Metolose 60SH-4000 (HPMC) was provided from Shin-Etsu Chemical Co., Ltd. (Tokyo, Japan). Hyaluronic acid was provided from SK Bioland (Cheonan, Korea). High-performance liquid chromatography (HPLC)-grade acetonitrile (ACN) was obtained from JT Baker (Phillipsburg, NJ, USA). Cyclosporine A (CsA) was purchased from Allergan Co., Ltd. (Dublin, Ireland). All other chemicals were commercial products of analytical or reagent grade and used without further purification.