Trypan blue dye solution

Manufactured by Merck Group

Sourced in United States

Trypan blue dye solution is a laboratory reagent used in cell counting and viability assays. It is a blue azo dye that selectively stains dead or damaged cells, allowing for the differentiation between live and non-viable cells under a microscope.

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Lab products found in correlation

Tween 20, by Merck Group (1 mentions) α tocopherol, by Merck Group (1 mentions) Rpmi 1640 with l gln, by Nacalai Tesque (1 mentions) Ascorbic acid, by Merck Group (1 mentions) 3 4 5 dimethylthiazol 2 yl 2 5 diphenyltetrazolium bromide mtt powder, by Merck Group (1 mentions) Penicillin streptomycin mixed solution stabilized, by Nacalai Tesque (1 mentions) Hplc grade methanol, by Merck Group (1 mentions) Penicillin streptomycin antibiotic, by GE Healthcare (1 mentions) Polysorbate 80, by Thermo Fisher Scientific (1 mentions) Lecithin, by Thermo Fisher Scientific (1 mentions) Sorbitol, by Merck Group (1 mentions) Trypsin edta, by GE Healthcare (1 mentions) Methyl thiazolyl tetrazolium (mtt), by Merck Group (1 mentions) Synergy h1 microplate reader, by Agilent Technologies (1 mentions)

Spelling variants of this product

Trypan blue (1976 citations) Trypan blue solution (510 citations) Trypan blue dye (211 citations) TM-blue (2 citations)

5 protocols using trypan blue dye solution

Evaluation of Antioxidant Potential and Cell Viability

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Chemicals were purchased from Fisher Scientific (Loughborough, Leicestershire, UK); RPMI-1640 with L-Gln (Roswell Park Memorial Institute Medium), penicillin-streptomycin mixed solution (stabilized) and 0.25%-trypsin solution were purchased from Nacalai Tesque Inc. (Kyoto, Japan); 2,2-diphenyl-2pocrylhydrazyl hydrate (DPPH), Folin-Ciocalteu’s phenol reagent, Tween 20, α-tocopherol, ascorbic acid, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) powder and trypan blue dye solution were purchased from Sigma-Aldrich (St. Louis, MO, USA); gallic acid was obtained from Fluka Biochemica (Buchs, Switzerland); fetal bovine serum (FBS) (EU approved) was purchased from Tico Europe Ltd. (Amstelveen, The Netherlands, Europe); dimethyl sulfoxide analytical grade (DMSO) and phosphate buffered saline tablet (PBS) was purchased from Millipore Sigma (Burlington, MA, USA).

Lee J.J., Saiful Yazan L., Kassim N.K., Che Abdullah C.A., Esa N., Lim P.C, & Tan D.C. (2020). Cytotoxic Activity of Christia vespertilionis Root and Leaf Extracts and Fractions against Breast Cancer Cell Lines. Molecules, 25(11), 2610.

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Cell Viability Assay with Trypan Blue

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Control or treated cells (1.5 × 10⁶/mL) were cultured in 48-well plates. After the incubation period (0–96 h), each sample was carefully resuspended, and a 10 μL aliquot was mixed with 10 μL of trypan blue dye solution (Sigma-Aldrich, Cat. #15250061). Then, 10 μL of the mixture was placed in a Neubauer chamber to count live (unstained) and dead (blue-stained) cells. Cell counts from at least three independent experiments were averaged and plotted. A Nikon D750 camera was adapted to a Nikon light microscope for cell counting to acquire representative images of cell cultures and treatments.

Olivas-Aguirre M., Pérez-Chávez J., Torres-López L., Hernández-Cruz A., Pottosin I, & Dobrovinskaya O. (2023). Dexamethasone-Induced Fatty Acid Oxidation and Autophagy/Mitophagy Are Essential for T-ALL Glucocorticoid Resistance. Cancers, 15(2), 445.

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Cytotoxicity Evaluation of Thymoquinone

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Hydrogenated palm oil (Softisan 154) was obtained from Condea (Witten, Germany). Olive oil (Basso) was obtained from Basso Fegele and Figli Srl (San Michele Di Serino, Italy). Eagle's minimal essential medium (EMEM), thymoquinone (TQ), 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) powder, trypan blue dye solution, propidium iodide (PI), thimerosal, and sorbitol were purchased from Sigma-Aldrich (St. Louis, USA). RPMI-1640 tissue culture medium, penicillin/streptomycin antibiotic, Mycoplex foetal bovine serum (FBS), and trypsin-EDTA were purchased from PAA Laboratories (Linz, Austria). Other reagents used were lecithin, a form of phospholipid (Cologne, Germany), nonionic surfactant Polysorbate 80 (Fisher-Scientific, USA), and HPLC grade methanol (Merck, USA).

Ng W.K., Saiful Yazan L., Yap L.H., Wan Nor Hafiza W.A., How C.W, & Abdullah R. (2015). Thymoquinone-Loaded Nanostructured Lipid Carrier Exhibited Cytotoxicity towards Breast Cancer Cell Lines (MDA-MB-231 and MCF-7) and Cervical Cancer Cell Lines (HeLa and SiHa). BioMed Research International, 2015, 263131.

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Cell Viability Assessment: MTT and Trypan Blue

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For the MTT assay, 100 μl of MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) solution (Sigma) was added to the cell cultures in 96-well plates and incubated at 37 °C with 5% CO₂ for 2 h. Viable cells transform the soluble MTT salt to insoluble blue formazan crystals. Formazan crystals formed were dissolved with 100 μl of solubilization buffer at 37 °C. The absorbance of the solubilized formazan was measured at an optical density (OD) of 570 nm (along with the reference OD at 650 nm) using a Synergy H1 Microplate Reader (BioTek). For the trypan blue exclusion assay, cells were trypsinized and stained with 0.4% trypan blue dye solution (Sigma). Viable cells were counted in four different fields of a hemocytometer under microscopy.

Wong A.S., Choi G.C., Cheng A.A., Purcell O, & Lu T.K. (2015). Massively parallel high-order combinatorial genetics in human cells. Nature biotechnology, 33(9), 952-961.

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In Vitro Breast Cancer Cell Assay

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Chemicals, reagents and equipment All the chemicals used in the experiment were of high quality and analytical grade. The chemicals procured for the experiment were methanol, ethyl acetate, hexane, chloroform, ethanol, acetone, 0.4% Trypan Blue dye solution (Sigma), Sulforhodamine B (SRB) solution, DMSO (Dimethyl sulphoxide), microscope (Carl Zeiss) and haemocytometer. Human breast cancer cells were maintained and all in vitro experiments were conducted at was conducted at ACTREC, Mumbai.

Shaikh A., Patel M.A., & Padul M.V. (2020). Anticancer Potential of Ficus religiosa (Linn) Against Human Breast Cancer Cell Line MDA-MB-231 and its Phytoconstituents by HR-LCMS.

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