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Nbp1 19412

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NBP1-19412 is a rabbit polyclonal antibody that recognizes the mitogen-activated protein kinase kinase 1 (MAP2K1) protein. MAP2K1 is a serine/threonine protein kinase that plays a key role in the extracellular signal-regulated kinase (ERK) signaling pathway.

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Lab products found in correlation

Nb100 1798, by Novus Biologicals (2 mentions) Ab110413, by Abcam (2 mentions) L7543, by Merck Group (2 mentions) Aconitase 1 antibody, by Novus Biologicals (2 mentions) Apg7 h 300 sc 33211, by Santa Cruz Biotechnology (2 mentions) Cd71 h 300 sc 9099, by Santa Cruz Biotechnology (2 mentions) A00158, by Merck Group (2 mentions) Mitoprofile total oxphos antibody, by Abcam (2 mentions) Sc 33682, by Santa Cruz Biotechnology (2 mentions) Sc 1797, by Santa Cruz Biotechnology (2 mentions) Asp175, by Cell Signaling Technology (2 mentions) Sc 7382, by Santa Cruz Biotechnology (2 mentions)

2 protocols using nbp1 19412

1

Immunoblotting Analyses of Lung Proteins

Check if the same lab product or an alternative is used in the 5 most similar protocols
Immunoblot analyses were performed in whole lung homogenates or mitochondrial-enriched fractions isolated from the lungs of mice using standard immunoblotting techniques with the following antibodies: Irp2 (1:1000 7H6: sc-33682, 1:1000 Santa Cruz, NB100-1798, Novus Biologicals and Irp2 antibody from Tracey Rouault at a 1:1000 dilution), Irp1 (1:1000 NBP1-19412, aconitase 1 Antibody, Novus Biologicals), LC3B (1:2000 L7543, Sigma Aldrich), Atg 7 (1:2000 APG7 (H-300): sc-33211, Santa Cruz), transferrin receptor 1 (1:1000 CD71 (H-300): sc-9099, Santa Cruz), actin (1:10,000 A00158, Sigma Aldrich), electron transport chain components including COX MTCO1 (1:500 MitoProfile® Total OXPHOS antibody, ab110413, Abcam), ferritin (1:1000 H-53: sc-25617, Santa Cruz), tom20 (1:2000 FL-145, sc-11415, Santa Cruz), frataxin (1:1000 H-155: sc-25820, Santa Cruz), mitoferrin 2 (1:1000 P-12: sc-138430, Santa Cruz), cleaved- caspase 3 (1:500 Asp175, Cell Signaling technologies), Bcl2 (1:1000 sc-7382, Santa Cruz), FBXL5 (1:1000 N0039, Neoclone Biotechnology) COX4I2 (1:1000 H00084701-M01 Abnova) and HO-1 (1:1000 sc-1797, Santa Cruz).
Cloonan S.M., Glass K., Laucho-Contreras M.E., Bhashyam A.R., Cervo M., Pabón M.A., Konrad C., Polverino F., Siempos I.I., Perez E., Mizumura K., Ghosh M.C., Parameswaran H., Williams N.C., Rooney K.T., Chen Z.H., Goldklang M.P., Yuan G.C., Moore S.C., Demeo D.L., Rouault T.A., D’Armiento J.M., Schon E.A., Manfredi G., Quackenbush J., Mahmood A., Silverman E.K., Owen C.A, & Choi A.M. (2016). Mitochondrial iron chelation ameliorates cigarette-smoke induced bronchitis and emphysema in mice. Nature medicine, 22(2), 163-174.
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2

Immunoblotting Analyses of Lung Proteins

Check if the same lab product or an alternative is used in the 5 most similar protocols
Immunoblot analyses were performed in whole lung homogenates or mitochondrial-enriched fractions isolated from the lungs of mice using standard immunoblotting techniques with the following antibodies: Irp2 (1:1000 7H6: sc-33682, 1:1000 Santa Cruz, NB100-1798, Novus Biologicals and Irp2 antibody from Tracey Rouault at a 1:1000 dilution), Irp1 (1:1000 NBP1-19412, aconitase 1 Antibody, Novus Biologicals), LC3B (1:2000 L7543, Sigma Aldrich), Atg 7 (1:2000 APG7 (H-300): sc-33211, Santa Cruz), transferrin receptor 1 (1:1000 CD71 (H-300): sc-9099, Santa Cruz), actin (1:10,000 A00158, Sigma Aldrich), electron transport chain components including COX MTCO1 (1:500 MitoProfile® Total OXPHOS antibody, ab110413, Abcam), ferritin (1:1000 H-53: sc-25617, Santa Cruz), tom20 (1:2000 FL-145, sc-11415, Santa Cruz), frataxin (1:1000 H-155: sc-25820, Santa Cruz), mitoferrin 2 (1:1000 P-12: sc-138430, Santa Cruz), cleaved- caspase 3 (1:500 Asp175, Cell Signaling technologies), Bcl2 (1:1000 sc-7382, Santa Cruz), FBXL5 (1:1000 N0039, Neoclone Biotechnology) COX4I2 (1:1000 H00084701-M01 Abnova) and HO-1 (1:1000 sc-1797, Santa Cruz).
Cloonan S.M., Glass K., Laucho-Contreras M.E., Bhashyam A.R., Cervo M., Pabón M.A., Konrad C., Polverino F., Siempos I.I., Perez E., Mizumura K., Ghosh M.C., Parameswaran H., Williams N.C., Rooney K.T., Chen Z.H., Goldklang M.P., Yuan G.C., Moore S.C., Demeo D.L., Rouault T.A., D’Armiento J.M., Schon E.A., Manfredi G., Quackenbush J., Mahmood A., Silverman E.K., Owen C.A, & Choi A.M. (2016). Mitochondrial iron chelation ameliorates cigarette-smoke induced bronchitis and emphysema in mice. Nature medicine, 22(2), 163-174.
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