Donkey anti sheep igg hrp secondary antibody

Manufactured by Santa Cruz Biotechnology

Donkey anti-sheep IgG-HRP secondary antibody is a reagent used in immunoassay techniques. It is an antibody that binds to sheep immunoglobulin G (IgG) and is conjugated with the enzyme Horseradish Peroxidase (HRP). This secondary antibody can be used to detect and quantify the presence of target proteins in samples.

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Lab products found in correlation

Supersignal west femto maximum sensitivity substrate, by Thermo Fisher Scientific (1 mentions) Chemidoc mp system, by Bio-Rad (1 mentions)

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Donkey anti-sheep HRP (2 citations)

2 protocols using donkey anti sheep igg hrp secondary antibody

Retinoic Acid-Induced Differentiation of SK-N-BE(2) Cells

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SK-N-BE(2) cells differentiated with 10 μm all-trans-retinoic acid for a period of 6 days were lysed in SDS sample buffer at a concentration of 2 × 10⁴ cell/μl. Lysates were separated on 12% acrylamide gels by SDS-PAGE. Samples were transferred onto a nitrocellulose membrane, blocked for 1 h at room temperature in 5% skim milk powder and labeled overnight with the following Tpm-specific sheep polyclonal antibodies: γ/9d (Merck Millipore, catalog no. AB5447), α/1b (Merck Millipore, catalog no. ABC499), and α/9d (Merck Millipore, catalog no. AB5441) (1 (link)). A donkey anti-sheep IgG-HRP secondary antibody (Santa Cruz Biotechnology, catalog no. sc-2473) was applied for 1 h at room temperature before HRP activity was developed using the SuperSignal^TM West Femto maximum sensitivity substrate (Thermo Fisher, catalog no. 34095). Membranes were imaged with a Bio-Rad ChemiDoc^TM MP system using Image La^TM software (Bio-Rad).

Pathan-Chhatbar S., Taft M.H., Reindl T., Hundt N., Latham S.L, & Manstein D.J. (2017). Three mammalian tropomyosin isoforms have different regulatory effects on nonmuscle myosin-2B and filamentous β-actin in vitro. The Journal of Biological Chemistry, 293(3), 863-875.

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Comprehensive Antibody Characterization for Tropomyosin Isoforms

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Antibodies used in this study include the mouse monoclonal antibody QIAexpress Penta⋅His, BSA–free (Qiagen, catalogue no. 34650); the Tpm-specific sheep polyclonal antibodies TPM3/9d (Merck Millipore, catalogue no. AB5447), TPM1/1b (Merck Millipore, catalogue no. ABC499), and TPM1/9d (Merck Millipore, catalogue no. AB5441); donkey anti–sheep IgG–HRP secondary antibody (Santa Cruz Biotechnology, catalogue no. sc–2473). Rabbit polyclonal antibody D55Ac was raised against the peptide Ac–MDAIKKKMQMLKLD (Eurogentec, Seraing, Belgium). D55Ac specifically detects N–acetylated isoforms derived from TPM1 and TPM2 that share the use of exon 1a. The epitope is found in Tpm1.1, Tpm1.2, Tpm1.3, Tpm1.4, Tpm1.5, Tpm1.6, Tpm1.7, Tpm1.10, Tpm2.1, Tpm2.2, Tpm2.3, and Tpm2.4.

Reindl T., Giese S., Greve J.N., Reinke P.Y., Chizhov I., Latham S.L., Mulvihill D.P., Taft M.H, & Manstein D.J. (2022). Distinct actin–tropomyosin cofilament populations drive the functional diversification of cytoskeletal myosin motor complexes. iScience, 25(7), 104484.

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