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3 protocols using ultra pure nitric acid

1

Iron Quantification and Chelation Assay

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All the chemicals used were of analytical reagent grade. Nitrilotriacetic acid (NTA), sodium thioglycollate (TGA), BPP3-morpholinopropane-1-sulfonic acid, 3-(N-morpholino) propane sulfonic acid (MOPS), ascorbic acid, sodium dithionite, holo-transferrin, DNA from calf thymus, bleomycin sulfate, magnesium chloride, thiobarbituric acid, butanol, hydrochloric acid, bovine serum albumin and magnesium chloride were purchased from Sigma Aldrich (St. Louis, MI, USA). Formic acid was procured from Fluka (St. Louis, MI, USA). Amicon® Ultra 0.5 mL filters (30 kDa) for protein purification and concentration were purchased from EMD Millipore (Billerica, MA, USA). Ultra-pure nitric acid was obtained from Fisher Scientific (Waltham, MA, USA). Ferrlecit®. Two different standard solutions of iron (Fe) (1000 ppm) were purchased from two separate sources Perkin Elmer (Shelton, CT, USA) and High Purity Standards (Charleston, SC, USA). A solution of 1000 ppm Indium (In) was purchased from Perkin Elmer (Shelton, CT, USA). Commercial drug product was obtained from a retail pharmacy.
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2

Photocrosslinkable Polymer-Fabric Composites

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The ZnTMPyP4+ tetrachloride PS was purchased from Frontier Scientific, while MB and RB were obtained from Acros Organics. The photocrosslinkable SbQ-PVA polymer with 4.1 mol% functional SbQ groups was supplied by Polysciences, Inc. The following materials were donated by Vescom America: Capri (polyester curtain fabric), Deans (polyester upholstery), Husk (proprietary wallcovering), PRU-86364 (proprietary wallcovering), and Wolin (a 75/20/5 w/w/w/ wool/polyester/polyamide upholstery combination). Buffer salts for the preparation of phosphate-buffered saline (PBS) solution and ultrapure nitric acid for ICP-OES analysis were purchased from Fisher Scientific. Tryptic soy broth was purchased from Teknova, and all media and buffer solutions were prepared in ultrapure water provided by an Easypure II system (Barnstead).
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3

Photocrosslinkable Polymer Composite Synthesis

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Titanium(IV) n-butoxide
(99+%, Alfa Aesar) and acetone (ACS reagent, Alfa Aesar) were purchased
from Fisher Scientific. Copper(II) acetylacetonate (97%) and octanoic
acid (≥98%) were purchased from Sigma-Aldrich. The photo-cross-linkable
SbQ-PVA polymer with 4.1 mol % functional SbQ groups was supplied
by Polysciences, Inc. Deionized water (18.2 MΩ cm) and dry ice
were sourced internally. YSZ milling media (3 mm, McMaster Carr) was
used for powder milling, and BYK 156 (BYK) was used as the milling
dispersant. All materials were used as received. Buffer salts for
the preparation of phosphate-buffered saline (PBS) solution and ultrapure
nitric acid for inductively coupled plasma-optical emission spectroscopy
(ICP-OES) analysis were purchased from Fisher Scientific. Tryptic
soy broth was purchased from Teknova, and all media and buffer solutions
were prepared in ultrapure water provided by an Easypure II system
(Barnstead).
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