A glass slide fixed with bacterial cells was first stained with crystal violet dye solution (Sigma) (2% w/v crystal violet dye in 95% v/v ethanol) for Gram staining. These slides were flooded with Gram’s iodine [1g iodine (Riedel-de Haen) and 2g potassium iodide (Merck) in 300ml distilled water] and counterstained with safranin (Merck) (2.5% w/v in 95% v/v ethanol; Beveridge, 2001 (link)). Samples were observed under a microscope at 100× and 1,000× magnification.
Iodine
Manufactured by Honeywell
Iodine is a chemical element used as a laboratory reagent. It is a solid at room temperature, with a distinctive violet-blue color. Iodine has a variety of applications in scientific and industrial settings, including as a disinfectant, oxidizing agent, and in the production of various chemicals.
Automatically generated - may contain errors
Lab products found in correlation
Safranin, by Merck Group (1 mentions)
Potassium iodide, by Merck Group (1 mentions)
Crystal violet dye solution, by Merck Group (1 mentions)
Ethyl cellulose, by Merck Group (1 mentions)
Titanium tetrachloride, by Merck Group (1 mentions)
Pedot pss, by Merck Group (1 mentions)
Tetraammineplatinum 2 chloride hydrate, by Merck Group (1 mentions)
Tert butylpyridine, by Merck Group (1 mentions)
1 propyl 3 methylimidazolium iodide, by Merck Group (1 mentions)
Lithium iodide anhydrous, by Thermo Fisher Scientific (1 mentions)
Acetonitrile, by Merck Group (1 mentions)
Lithium carbonate, by Merck Group (1 mentions)
Isopropyl alcohol, by Merck Group (1 mentions)
Tert butanol, by Merck Group (1 mentions)
Ruthenizer 535 bistba, by Solaronix (1 mentions)
2 protocols using iodine
1
Gram Staining of Bacterial Cells
2
Fabrication of Dye-Sensitized Solar Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Cantaloupe was purchased from a local market. The fly ash powder utilized in the present investigation was obtained from the Mae Moh Power Plant in the northern Thai province of Lampang. PEDOT:PSS, titanium tetrachloride (TiCl4), tetraammineplatinum (II) chloride hydrate (Pt (NH3)4Cl2·xH2O), ethyl cellulose (C20H38O11), isopropyl alcohol (>99.8 %, C3H8O), tert-butanol (>99.70 %, C4H10O), 1-propyl-3-methylimidazolium iodide (>98 %, C16H31IN2), lithium carbonate (99.99 %, Li2CO3), tert-butylpyridine (96 %, C13H21N), and acetonitrile (99.8 %, CH3CN) were obtained from Sigma-Aldrich. Ruthenizer 535-bisTBA and PST-18NR paste were acquired from Solaronix S.A. Lithium iodide anhydrous (99.99 %, Lil) and lithium perchlorate anhydrous (99 %, LiClO4) were obtained from Alfa Aesar. Iodine (>99.8 %, I2) was acquired from Riedel-de Haen. Fluorine-doped tin oxide glass (FTO, 15 Ω/sq) was obtained from Solaronix. All chemicals were used as received with no further purification.
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