Nickel(II) Chloride was obtained from Wako Pure Chemical Corporation. Freund’s incomplete adjuvant (IFA) and complete adjuvant (CFA) were from MP Biomedicals. The primary antibody specific for Sema3A was from Abcam. Antibodies specific for phospho-p38 (Thr180/Tyr182) and p38 were obtained from Cell Signaling Technology. Rabbit antibody to GAPDH was obtained from Osenses. Rabbit antibody to β-actin was obtained from Bioss. Horseradish peroxidase (HRP)-conjugated anti-rabbit secondary antibody were obtained from Cell Signaling. FITC anti-mouse CD11c, Alexa Fluor 488 anti-mouse CD11b, PE anti-mouse F4/80, PE anti-mouse I-A/I-E, FITC anti-mouse CD3, PE/Cyanine7 anti-mouse CD4 and PE anti-mouse CD8a were purchased from BioLegend. APC-Cy™7 Rat Anti-Mouse CD45 and Purified Rat Anti-Mouse CD16/CD32 (Mouse BD Fc Block™) were from BD Biosciences. 7-AAD Viability Staining Solution was from Invitrogen. Anti-rabbit Alexa Fluor 488 secondary antibody was from Abcam. ReadidropTM Propidium Iodide was from Bio-Rad Laboratories. The recombinant mouse Sema3A were purchased from R&D Systems.
Freund s incomplete adjuvant ifa
Manufactured by MP Biomedicals
Freund's incomplete adjuvant (IFA) is a water-in-oil emulsion used as an immunological adjuvant to enhance the immune response to an antigen. It consists of mineral oil and surfactant agents without the addition of mycobacterial components present in Freund's complete adjuvant. IFA is used in immunological research to potentiate the immune response during the production of antibodies.
Automatically generated - may contain errors
Lab products found in correlation
Pe anti mouse 1 a 1 e, by BioLegend (2 mentions)
Complete adjuvant cfa, by MP Biomedicals (2 mentions)
Antibodies specific for phospho p38 thr180 tyr182 and p38, by Cell Signaling Technology (2 mentions)
Alexa fluor 488 anti mouse cd11b, by BioLegend (2 mentions)
Nickel 2 chloride, by Fujifilm (2 mentions)
Pe anti mouse f4 80, by BioLegend (2 mentions)
Horseradish peroxidase hrp conjugated anti rabbit secondary antibody, by Cell Signaling Technology (2 mentions)
7 aad viability staining solution, by Thermo Fisher Scientific (1 mentions)
Purified rat anti mouse cd16 cd32 mouse fc block, by BD (1 mentions)
Fitc anti mouse cd3, by BioLegend (1 mentions)
Pe anti mouse cd8a, by BioLegend (1 mentions)
Fitc anti mouse cd11c, by BioLegend (1 mentions)
Apc cy7 rat anti mouse cd45, by BD (1 mentions)
Alexa fluor 488 secondary antibody, by Abcam (1 mentions)
Readidrop propidium iodide, by Bio-Rad (1 mentions)
Pe cyanine7 anti mouse cd4, by BioLegend (1 mentions)
Alexa fluor 647 antimouse cd206 mmr, by BioLegend (1 mentions)
Alexa fluor 488 antimouse cd11c, by BioLegend (1 mentions)
Alexa fluor 488 anti rabbit secondary antibody, by Abcam (1 mentions)
Pe anti mouse cd115, by BioLegend (1 mentions)
2 protocols using freund s incomplete adjuvant ifa
1
Sema3A Signaling in Immune Responses
2
Sema3A Regulation and Immune Response
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Nickel(II) Chloride was obtained from Wako Pure Chemical Corporation. Freund's incomplete adjuvant (IFA) and complete adjuvant (CFA) were from MP Biomedicals. The primary antibodies specific for Sema3A were from Bioss Antibodies (for Western blotting) and Abcam (for immunohistochemistry/immunocytochemistry), respectively. Antibodies specific for phospho-p38 (Thr180/Tyr182) and p38 were obtained from Cell Signaling Technology. Antibody specific for mouse TSLP was from Abcam. Rabbit antibody to GAPDH was obtained from Osenses. Alexa Fluor 488 anti-mouse CD11c, Alexa Fluor 488 anti-mouse CD11b, PE anti-mouse CD115, PE anti-mouse F4/80, PE anti-mouse I-A/I-E and Alexa Fluor 647 anti-mouse CD206 (MMR) were purchased from BioLegend. Horseradish peroxidase (HRP)-conjugated anti-rabbit secondary antibody were obtained from Cell Signaling. Anti-rabbit Alexa Fluor 488 secondary antibody was from Abcam.
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