Phospho c src

Manufactured by Cell Signaling Technology

Phospho-c-Src is a laboratory product from Cell Signaling Technology that detects the phosphorylation of the c-Src protein. It is a tool for researchers to analyze the activation state of the c-Src signaling pathway.

Automatically generated - may contain errors

Lab products found in correlation

Enhanced chemiluminescence ecl western blot detection system, by GE Healthcare (1 mentions) L798106, by Santa Cruz Biotechnology (1 mentions) Anti glyceraldehyde 3 phosphate dehydrogenase gapdh, by GeneTex (1 mentions) Cox 2 antibody, by Cell Signaling Technology (1 mentions) Dulbecco s modified eagle s medium dmem f 12 medium, by Thermo Fisher Scientific (1 mentions) Hybond c membrane, by GE Healthcare (1 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions) Trizol, by Thermo Fisher Scientific (1 mentions) U0126, by Santa Cruz Biotechnology (1 mentions) Phospho jak2, by Cell Signaling Technology (1 mentions) Phospho erk1 2, by Cell Signaling Technology (1 mentions) Phospho stat3, by Cell Signaling Technology (1 mentions) Hybond, by Cytiva (1 mentions) Phospho iκb, by Cell Signaling Technology (1 mentions) Pvdf membrane, by Bio-Rad (1 mentions) Enhanced chemi luminescence (ecl), by GE Healthcare (1 mentions) β actin, by Merck Group (1 mentions) Goat anti rabbit igg, by Promega (1 mentions) Hrp conjugated goat anti mouse igg, by Promega (1 mentions) Cre recombinase, by Fortrea (1 mentions)

Spelling variants of this product

Phospho-Src (39 citations) C-Src (20 citations) Anti-phospho-c-Src (6 citations)

4 protocols using phospho c src

Molecular Signaling Pathway Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

Dulbecco’s modified Eagle’s medium (DMEM)/F-12 medium, fetal bovine serum (FBS), and TRIzol were from Invitrogen (Carlsbad, CA). Hybond C membrane and enhanced chemiluminescence (ECL) Western blot detection system were from GE Healthcare Biosciences (Buckinghamshire, UK). Phospho-c-Src (Cat# 6943), phospho-Jak2 (Cat# 3776), phospho-ERK1/2 (Cat# 4370), phospho-STAT3 (Cat# 9145), COX-2 antibody (Cat# 12,282) antibodies were from Cell Signaling (Danver, MA). Anti-glyceraldehyde-3-phosphate dehydrogenase (GAPDH, Cat# GTX627408) antibody was from GeneTex (Irvine, CA, USA). Celecoxib (CLC), AG490, U0126, cucurbitacin E (CBE), Sc-19220, L798-106, and GW627368 were from Santa Cruz (Santa Cruz, CA). PP1 was from Biomol (Plymouth Meeting, PA). Bicinchoninic acid (BCA) protein assay reagent was from Pierce (Rockford, IL). Bradykinin (BK), enzymes, and other chemicals were from Sigma (St. Louis, MO).

Lee T.H., Liu P.S., Tsai M.M., Chen J.L., Wang S.J, & Hsieh H.L. (2020). The COX-2-derived PGE2 autocrine contributes to bradykinin-induced matrix metalloproteinase-9 expression and astrocytic migration via STAT3 signaling. Cell Communication and Signaling : CCS, 18, 185.

+ Open protocol

+ Expand

Western Blot Analysis of Signaling Proteins

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cells were washed with ice-cold PBS, and proteins were extracted at 4°C with M-PER, an inhibitor cocktail. Equivalent amounts of protein were subjected to SDS-PAGE with 7.5–15% Tris-Glycin gradient gel or 15% Tris-Glycin gel and transferred onto PVDF membranes (Bio-Rad Laboratories, Inc.). After blocking with 6% milk/TBS-T, membranes were incubated with primary antibodies to Stat5a, Stat5b, RNA polymerase (Santa Cruz Biotechnology, Inc.), Cre recombinase (Covance), phospho-Stat5, cleaved caspase-3, ERK1/2, phospho-ERK1/2, JNK, phospho-JNK, p38, phospho-p38, phospho-Akt, Akt, phospho-IκB, IκB, phospho–c-Src (Cell Signaling Technology), c-Src (EMD Millipore), and β-actin (Sigma-Aldrich), followed by HRP-conjugated goat anti–mouse IgG and goat anti–rabbit IgG (Promega). Immunoreactive bands were visualized with ECL (GE Healthcare) according to the manufacturer’s instructions. The blots were stripped by incubating for 20 min in stripping buffer (2% SDS, 100 mM 2-mercaptoethanol, and 62.5 mM Tris-HCl, pH 6.7) at 50°C and then were reprobed with other antibodies.

Hirose J., Masuda H., Tokuyama N., Omata Y., Matsumoto T., Yasui T., Kadono Y., Hennighausen L, & Tanaka S. (2014). Bone resorption is regulated by cell-autonomous negative feedback loop of Stat5–Dusp axis in the osteoclast. The Journal of Experimental Medicine, 211(1), 153-163.

+ Open protocol

+ Expand

TMS and trans-resveratrol Pharmacological Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols

TMS and trans-resveratrol were purchased from Cayman Chemical (Ann Arbor, MI). DHE was from Invitrogen™ (Carlsbad, CA), the CYP1B1 antibody was from BD Biosciences (Franklin Lakes, NJ), and antibodies against α-smooth muscle-specific actin, ERK1/2, p38 MAPK, c-Src, and Akt were purchased from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA). The primary phospho ERK1/2, phospho p38 MAPK, phospho c-Src, and phospho Akt anti-bodies were purchased from Cell Signaling Technology, Inc. (Danvers, MA). All other chemicals were purchased from Sigma (St. Louis, MO).

Jennings B.L., Montanez D.E., May ME J.r., Estes A.M., Fang X.R., Yaghini F.A., Malik K.U, & Kanu A. (2014). Cytochrome P450 1B1 contributes to increased blood pressure and cardiovascular and renal dysfunction in spontaneously hypertensive rats. Cardiovascular drugs and therapy / sponsored by the International Society of Cardiovascular Pharmacotherapy, 28(2), 145-161.

+ Open protocol

+ Expand

Antibody Generation and Characterization

Check if the same lab product or an alternative is used in the 5 most similar protocols

The rabbit polyclonal antibody against HK1 was generated by immunizing rabbits with His-fusion or GST-fusion proteins of human HK1 containing fragments (aa 316–410). The HK1-p-Y732 and HK2-p-Y686 polyclonal antibodies were raised by immunizing rabbits with phospho-peptide VDE{pTyr}SLNAGKQRYEC and GTGSNAC{pTyr}MEEMR, respectively. Mouse anti-HA (clone number F-7, 1:1,000), anti-Myc (clone number 9E10, 1:1,000), anti-c-Src (clone number B-12, 1:1,000) and rabbit anti-HA (clone number Y-11, 1:1,000) were purchased from Santa Cruz. Rabbit anti-HK1 (clone number C35C4, 1:1,000), anti-HK2 (clone number C64G5, 1:1,000), phospho-c-Src (clone number D49G4, 1:1,000) and mouse anti-p-Tyr (catalog number #9411, 1:1,000) were purchased from Cell Signaling Technology. Mouse anti-FLAG (M₂) (catalog number M8823, 1:5,000), anti-bromodeoxyuridine (clone number BMC9318, 0.2 μg per test) and anti-β-actin (clone number AC-15, 1:5,000) antibodies were purchased from Sigma. Deoxy-D-glucose 2-[1, 2-³H] (NET549250UC) and Glucose D-[6-¹⁴C] (NEC045X050UC) were purchased from PerkinElmer. 2-¹³C-glucose was purchased from Sigma. Various peptides were attained from Gen script (Nanjing) Company.

Zhang J., Wang S., Jiang B., Huang L., Ji Z., Li X., Zhou H., Han A., Chen A., Wu Y., Ma H., Zhao W., Zhao Q., Xie C., Sun X., Zhou Y., Huang H., Suleman M., Lin F., Zhou L., Tian F., Jin M., Cai Y., Zhang N, & Li Q. (2017). c-Src phosphorylation and activation of hexokinase promotes tumorigenesis and metastasis. Nature Communications, 8, 13732.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!