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Fibrinogen pbs solution

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Fibrinogen-PBS solution is a laboratory reagent composed of fibrinogen dissolved in phosphate-buffered saline (PBS). It is a colorless, transparent liquid intended for use in various in vitro research and analytical applications.

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Lab products found in correlation

Thrombin, by Merck Group (2 mentions) Amg evos xl core digital microscope, by Thermo Fisher Scientific (2 mentions) Sunitinib, by Cell Signaling Technology (2 mentions) Graphad prism, by GraphPad (2 mentions) Vatalanib, by Santa Cruz Biotechnology (2 mentions) Ebm 2, by Lonza (2 mentions)

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Fibrinogen (463 citations) PBS solution (57 citations) Fibrinogen solution (22 citations)

2 protocols using fibrinogen pbs solution

1

Inhibition of Angiogenic Sprouting in HUVEC Spheroids

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HUVEC were trypsinised and resuspended in EBM2 (Lonza) containing 2% foetal calf serum (FCS) at a concentration of 2.5×104 cells/ml. Methylcellulose was added at 0.25% (w/v) and 20μl drops were seeded in non-adherent dish. After inversion of the plate, suspended cells form a single spheroid containing approximately 500 cells. Treated spheroids were incubated with 1μM Sunitinib (Cell Signaling Technology, Inc) or 100nM Vatalanib (Santa Cruz) for 1hr prior to being embedded in a fibrin gel using 2.5mg/ml fibrinogen-PBS solution (Sigma) and 0.007 units of thrombin (Sigma). Once clotting occurred, EBM-2 containing 2% FCS was added with or without 1μM Sunitinib or 100nM Vatalanib. Spheroid photographs were taken after 24 hours using an AMG Evos XL Core digital microscope (Fisher Scientific, Loughborough, UK). Sprouting area was measured using ImageJ64 software and the results were analysed using Graphad Prism version 6 (Graphpad Software).
Behjati S., Tarpey P.S., Sheldon H., Martincorena I., Van Loo P., Gundem G., Wedge D.C., Ramakrishna M., Cooke S.L., Pillay N., Vollan H.K., Papaemmanuil E., Koss H., Bunney T.D., Hardy C., Joseph O.R., Martin S., Mudie L., Butler A., Teague J.W., Patil M., Steers G., Cao Y., Gumbs C., Ingram D., Lazar A.J., Little L., Mahadeshwar H., Protopopov A., Al Sannaa G.A., Seth S., Song X., Tang J., Zhang J., Ravi V., Torres K.E., Khatri B., Halai D., Roxanis I., Baumhoer D., Tirabosco R., Amary M.F., Boshoff C., McDermott U., Katan M., Stratton M.R., Futreal P.A., Flanagan A.M., Harris A, & Campbell P.J. (2014). Recurrent PTPRB and PLCG1 mutations in angiosarcoma. Nature genetics, 46(4), 376-379.
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2

Inhibition of Angiogenic Sprouting in HUVEC Spheroids

Check if the same lab product or an alternative is used in the 5 most similar protocols
HUVEC were trypsinised and resuspended in EBM2 (Lonza) containing 2% foetal calf serum (FCS) at a concentration of 2.5×104 cells/ml. Methylcellulose was added at 0.25% (w/v) and 20μl drops were seeded in non-adherent dish. After inversion of the plate, suspended cells form a single spheroid containing approximately 500 cells. Treated spheroids were incubated with 1μM Sunitinib (Cell Signaling Technology, Inc) or 100nM Vatalanib (Santa Cruz) for 1hr prior to being embedded in a fibrin gel using 2.5mg/ml fibrinogen-PBS solution (Sigma) and 0.007 units of thrombin (Sigma). Once clotting occurred, EBM-2 containing 2% FCS was added with or without 1μM Sunitinib or 100nM Vatalanib. Spheroid photographs were taken after 24 hours using an AMG Evos XL Core digital microscope (Fisher Scientific, Loughborough, UK). Sprouting area was measured using ImageJ64 software and the results were analysed using Graphad Prism version 6 (Graphpad Software).
Behjati S., Tarpey P.S., Sheldon H., Martincorena I., Van Loo P., Gundem G., Wedge D.C., Ramakrishna M., Cooke S.L., Pillay N., Vollan H.K., Papaemmanuil E., Koss H., Bunney T.D., Hardy C., Joseph O.R., Martin S., Mudie L., Butler A., Teague J.W., Patil M., Steers G., Cao Y., Gumbs C., Ingram D., Lazar A.J., Little L., Mahadeshwar H., Protopopov A., Al Sannaa G.A., Seth S., Song X., Tang J., Zhang J., Ravi V., Torres K.E., Khatri B., Halai D., Roxanis I., Baumhoer D., Tirabosco R., Amary M.F., Boshoff C., McDermott U., Katan M., Stratton M.R., Futreal P.A., Flanagan A.M., Harris A, & Campbell P.J. (2014). Recurrent PTPRB and PLCG1 mutations in angiosarcoma. Nature genetics, 46(4), 376-379.
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