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Anti human cd16 magnetic beads

Manufactured by Miltenyi Biotec
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Anti-human CD16 magnetic beads are a laboratory product used for the isolation and enrichment of CD16+ cells from human samples. The beads are coated with an anti-human CD16 antibody, which allows for the specific targeting and separation of CD16-expressing cells, such as natural killer cells and a subset of monocytes. This product can be utilized in various cell biology applications that require the isolation or purification of CD16-positive cell populations.

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Lab products found in correlation

Recombinant human m csf, by Thermo Fisher Scientific (2 mentions) Histopaque 1077, by Merck Group (2 mentions) Anti human cd14 magnetic beads, by Miltenyi Biotec (2 mentions) Sodium heparin vacutainer, by BD (2 mentions)

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Magnetic beads (547 citations)

2 protocols using anti human cd16 magnetic beads

1

Isolation and Stimulation of Human Monocyte-Derived Macrophages

Check if the same lab product or an alternative is used in the 5 most similar protocols
Venous blood was collected in Sodium-Heparin vacutainers (Cat# 367874, BD). Plasma and blood cells were separated using Histopaque-1077 (Cat# 10771, Sigma) according to the manufacturer’s protocol. Briefly, whole blood was diluted 1:1 with sterile DPBS-2 mM EDTA (Sigma or Corning) and overlaid (30 mL) on to 10 mL of Histopaque-1077. Gradients were centrifuged at 500xg for 30 min. The peripheral blood mononuclear cell (PBMC) layer at the plasma-Histopaque interface was transferred to a new tube and washed twice with cold DBPS-EDTA. PBMCs were isolated and CD16+ cells depleted using anti-human CD16 magnetic beads (Cat# 130-045-701, Miltenyi) using manufacturer’s protocol. After CD16 depletion, CD14+ monocytes were purified using anti-human CD14 magnetic beads (Cat# 130-050-201, Miltenyi). Monocytes were plated in 24-well tissue culture treated plates at density 5x105 per well in 1 mL of cRPMI supplemented with human recombinant M-CSF (50 ng/ml; Peprotech). Every two days the media was exchanges for fresh cRPMI containing M-CSF. At the day 7-8 the media was changed to cRPMI without M-CSF and stimulations were carried out as indicated. MoDMs were stimulated with LPS (100 ng/ml) and ATP (5 mM, 45 min).
Bambouskova M., Potuckova L., Paulenda T., Kerndl M., Mogilenko D.A., Lizotte K., Swain A., Hayes S., Sheldon R.D., Kim H., Kapadnis U., Ellis A.E., Isaguirre C., Burdess S., Laha A., Amarasinghe G.K., Chubukov V., Roddy T.P., Diamond M.S., Jones R.G., Simons D.M, & Artyomov M.N. (2021). Itaconate confers tolerance to late NLRP3 inflammasome activation. Cell reports, 34(10), 108756.
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2

Isolation and Stimulation of Human Monocyte-Derived Macrophages

Check if the same lab product or an alternative is used in the 5 most similar protocols
Venous blood was collected in Sodium-Heparin vacutainers (Cat# 367874, BD). Plasma and blood cells were separated using Histopaque-1077 (Cat# 10771, Sigma) according to the manufacturer’s protocol. Briefly, whole blood was diluted 1:1 with sterile DPBS-2 mM EDTA (Sigma or Corning) and overlaid (30 mL) on to 10 mL of Histopaque-1077. Gradients were centrifuged at 500xg for 30 min. The peripheral blood mononuclear cell (PBMC) layer at the plasma-Histopaque interface was transferred to a new tube and washed twice with cold DBPS-EDTA. PBMCs were isolated and CD16+ cells depleted using anti-human CD16 magnetic beads (Cat# 130-045-701, Miltenyi) using manufacturer’s protocol. After CD16 depletion, CD14+ monocytes were purified using anti-human CD14 magnetic beads (Cat# 130-050-201, Miltenyi). Monocytes were plated in 24-well tissue culture treated plates at density 5x105 per well in 1 mL of cRPMI supplemented with human recombinant M-CSF (50 ng/ml; Peprotech). Every two days the media was exchanges for fresh cRPMI containing M-CSF. At the day 7-8 the media was changed to cRPMI without M-CSF and stimulations were carried out as indicated. MoDMs were stimulated with LPS (100 ng/ml) and ATP (5 mM, 45 min).
Bambouskova M., Potuckova L., Paulenda T., Kerndl M., Mogilenko D.A., Lizotte K., Swain A., Hayes S., Sheldon R.D., Kim H., Kapadnis U., Ellis A.E., Isaguirre C., Burdess S., Laha A., Amarasinghe G.K., Chubukov V., Roddy T.P., Diamond M.S., Jones R.G., Simons D.M, & Artyomov M.N. (2021). Itaconate confers tolerance to late NLRP3 inflammasome activation. Cell reports, 34(10), 108756.
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