168 detector

Manufactured by Beckman Coulter

The 168 Detector is a laboratory equipment designed to detect and measure the intensity of light signals. It is a versatile instrument that can be used in various analytical applications. The core function of the 168 Detector is to convert light signals into electrical signals, which can then be processed and analyzed by connected systems or software.

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Lab products found in correlation

System gold 126 solvent module, by Beckman Coulter (3 mentions) Carry 4000 spectrophotometer, by Agilent Technologies (2 mentions) 2475 multi λ fluorescence detector, by Waters Corporation (1 mentions) Spherisorb 5 μm ods2, by Waters Corporation (1 mentions)

Close spelling variants of this product

168 UV–VIS detector (3 citations) System Gold 168 Diode array detector (3 citations) SYSTEM GOLD® 168 Detector (3 citations) 168 UV detector (2 citations)

4 protocols using 168 detector

Photosynthetic Pigment Analysis Protocol

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The Chl a/b ratio and Chl/carotenoid ratio were determined from the absorption spectra of 80% acetone extracts. The absorption spectra were fitted with the spectra of individual pigments in the same solvent, as described in Croce, Canino, Ros, and Bassi (2002). The quantification of different carotenoids was performed by HPLC using a System Gold 126 Solvent module and 168 Detector (Beckman Coulter) as described by Gilmore and Yamamoto (1991) with the modification reported in Xu, Tian, Kloz, and Croce (2015).

Bielczynski L.W., Schansker G, & Croce R. (2020). Consequences of the reduction of the Photosystem II antenna size on the light acclimation capacity of Arabidopsis thaliana. Plant, Cell & Environment, 43(4), 866-879.

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Chlorophyll and Carotenoid Quantification

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The amount of chlorophylls on a leaf fresh weight basis, Chl a/b ratio and chlorophyll/carotenoid (Chl/Car) ratio were determined from absorption spectra of 80% acetone extracts measured with a Carry 4000 spectrophotometer (Varian). The absorption spectra were fitted with the spectra of individual pigments in the same solvent, as described in Croce et al. (2002) (link). The quantification of different carotenoids was performed by HPLC using a System Gold 126 Solvent module and 168 Detector (Beckman Coulter) as described by Gilmore and Yamamoto (1991) (link) with the modification reported in Xu et al. (2015) (link).

Bielczynski L.W., Schansker G, & Croce R. (2016). Effect of Light Acclimation on the Organization of Photosystem II Super- and Sub-Complexes in Arabidopsis thaliana. Frontiers in Plant Science, 7, 105.

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Quantifying Bone Collagen Crosslinks via HPLC

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From each paired sample, a ~2.5 mm x 2.5 mm corner section was cut for high performance liquid chromatography (HPLC). Bone segments were demineralized in 20% ethylenediaminetetraacetic acid (EDTA) at 4 °C. Then, these bone segments were hydrolyzed in 6 N HCl with 4.5 mM alpha-amino-N-butyric acid (α-ABA) for ~20 hours. After removal of the acid and filtration through 0.2 µm syringe filter, a portion of the re-suspended hydrolysate was used to measure hydroxyproline (to give mole of collagen) via the chromatogram generated by a UV detector (Beckman Coulter 168 Detector, Brea, CA) and following our previously published HPLC method (56 (link)).
To determine crosslink concentrations, another portion of the re-suspended hydrolysate was diluted in acetonitrile-heptafluorobutyric acid solution (10% v/v) with 0.25 µg/ml of pyridoxine (PYR) as the internal standard. Samples were injected along with standards consisting of PYR and varying concentrations of pyridinoline (PYD), deoxypyridinoline (DPD) and pentosidine (PEN) into a silica-based, reversed-phase C18 column (Waters Spherisorb® 5μm ODS2, Milford, MA)(57 (link)). A programmable fluorescence detector (Waters 2475 Multi λ Fluorescence Detector) was used to generate chromatograms. Moles of each crosslink per sample were divided by the corresponding moles of collagen.

Unal M., Uppuganti S., Leverant C.J., Creecy A., Granke M., Voziyan P, & Nyman J.S. (2018). Assessing Glycation-mediated Changes in Human Cortical Bone with Raman Spectroscopy. Journal of biophotonics, 11(8), e201700352.

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Photosynthetic Pigment Quantification

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The Chl a/b ratio and Chl/Car ratio were determined from absorption spectra of 80% acetone extracts measured with a Carry 4000 spectrophotometer (Varian). The absorption spectra were fitted with the spectra of individual pigments in the same solvent, as previously described (Croce et al. 2002 (link)). The quantification of different carotenoids was performed by HPLC using a System Gold 126 Solvent module and 168 Detector (Beckman Coulter) as previously described in (Gilmore and Yamamoto 1991 (link)) with modifications (Xu et al. 2015 (link)).

Bielczynski L.W., Xu P, & Croce R. (2022). PSII supercomplex disassembly is not needed for the induction of energy quenching (qE). Photosynthesis Research, 152(3), 275-281.

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