Tobramycin sulfate, moxifloxacin hydrochloride, rifampicin, and ciprofloxacin hydrochloride were obtained from AK Scientific, Inc. (Union City, CA, USA). fosfomycin sodium was obtained from Sigma-Aldrich (St. Louis, MO, USA). Glucose-6-phosphate (Sigma-Aldrich) was added to the medium at a final concentration of 25 mg/L for all evaluations of fosfomycin.
Fosfomycin sodium
Manufactured by Merck Group
Sourced in United States, Denmark
Fosfomycin sodium is a broad-spectrum antibiotic agent. It inhibits an early stage of bacterial cell wall synthesis.
Automatically generated - may contain errors
Lab products found in correlation
Glucose 6 phosphate (g6p), by Merck Group (1 mentions)
Fosfomycin, by Merck Group (1 mentions)
Caffeic acid, by Merck Group (1 mentions)
Chlorogenic acid, by Merck Group (1 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (1 mentions)
Metronidazole, by Merck Group (1 mentions)
Metronidazole, by B. Braun (1 mentions)
3 protocols using fosfomycin sodium
1
Antimicrobial Agents Evaluation
2
Preparation of Antibacterial Compound Solutions
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The
50 mg/mL fosfomycin sodium (analytical standard, Sigma-Aldrich Inc.,
St. Louis, MO) stock solution was prepared by dissolving fosfomycin
sodium in distilled water. The chemicals, chlorogenic acid, and caffeic
acid, were identified by the ligand–protein docking (as shown
in Section 3.1). The chlorogenic acid (≥95%, Sigma-Aldrich
Inc., St. Louis, MO) stock solution was prepared by dissolving the
chemical in DMSO (Sigma-Aldrich Inc., St. Louis, MO) with a final
concentration of 60 mg/mL. The caffeic acid stock solution was prepared
by dissolving caffeic acid (≥98%, Sigma-Aldrich Inc., St. Louis,
MO) in DMSO with the final concentration of 50 mg/mL. All stock solutions
were sterilized using a 0.22 μm syringe filter and stored under
−20 °C until further use.
50 mg/mL fosfomycin sodium (analytical standard, Sigma-Aldrich Inc.,
St. Louis, MO) stock solution was prepared by dissolving fosfomycin
sodium in distilled water. The chemicals, chlorogenic acid, and caffeic
acid, were identified by the ligand–protein docking (as shown
in Section 3.1). The chlorogenic acid (≥95%, Sigma-Aldrich
Inc., St. Louis, MO) stock solution was prepared by dissolving the
chemical in DMSO (Sigma-Aldrich Inc., St. Louis, MO) with a final
concentration of 60 mg/mL. The caffeic acid stock solution was prepared
by dissolving caffeic acid (≥98%, Sigma-Aldrich Inc., St. Louis,
MO) in DMSO with the final concentration of 50 mg/mL. All stock solutions
were sterilized using a 0.22 μm syringe filter and stored under
−20 °C until further use.
3
Quantifying Antimicrobial Drug Levels
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The drugs used in this investigation were the following: fosfomycin 2 g (Infectofos, Infectopharm, Germany) dissolved in sterile water for injections (Sterilt Vand "SAD", Denmark), metronidazole 5 mg/ml (metronidazole "B. Braun", Denmark), and rhGM-CSF 0.25 µg/µl (Repomol, Reponex Pharmaceuticals, Denmark). We used a total of 4 g fosfomycin, 1 g metronidazole, and 50 µg rhGM-CSF. To optimise the parameters for High Pressure Liquid Chromatography-Mass Spectrometry (HPLC-MS) pure analytical standards were used. These were fosfomycin sodium (Sigma-Aldrich, Glostrup, Denmark) and metronidazole (Sigma-Aldrich, Glostrup, Denmark). We used the internal standards (IS) ethylphophonic acid (Sigma-Aldrich, Glostrup, Denmark) and metronidazole-d 4 (Toronto Research Chemicals, ON, Canada).
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