Labelling of RNA was done as recommended by Agilent Technologies using the One-Color Microarray-Based Gene Expression Analysis Low Input Quick Amp Labelling. The input was 10 ng of total RNA and 600 ng of labelled cRNA was used on the eight-pack array. Hybridization was performed as described in the One-Color Microarray-Based Gene Expression Analysis Low Input Quick Amp Labelling protocol from Agilent in the hybridization oven (G2545A hybridization Oven Agilent Technologies). The hybridization temperature was 65 °C with rotation speed 10 r/min for 17 h. After 17 h the arrays were washed as described in the One-Color Microarray-Based Gene Expression Analysis Low Input Quick Amp Labelling protocol from Agilent. The arrays were scanned using the DNA microarray scanner with Surescan high resolution Technology from Agilent Technologies. Agilent Scan Control with resolution of 5 μm, 16 bits and PMT of 100%. Feature extraction was performed using protocol 10.7.3.1 (v10.7) for one colour gene expression. Geo accession number GSE94370 (https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE94370 ).
One color microarray based gene expression analysis low input quick amp labelling protocol
Manufactured by Agilent Technologies
The One-Color Microarray-Based Gene Expression Analysis Low Input Quick Amp Labelling protocol is a laboratory technique used for gene expression analysis. It allows for the labeling and amplification of RNA samples with low input amounts, enabling the study of gene expression profiles using microarray technology.
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2 protocols using one color microarray based gene expression analysis low input quick amp labelling protocol
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One-Color Microarray-Based Gene Expression Analysis
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One-Color Microarray-Based Gene Expression Analysis
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Labeling of RNA was done as recommended by Agilent Technologies using the One-Color Microarray-Based Gene Expression Analysis Low input Quick Amp Labelling. The input was 10 ng of total RNA, and 600 ng of labeled complementary RNA was used on the 8-pack array. Hybridization was performed as described in the One-Color Microarray-Based Gene Expression Analysis Low input Quick Amp Labelling protocol from Agilent in the hybridization oven (G2545A hybridization Oven Agilent Technologies). The hybridization temperature is 65 °C with rotation speed 10 rpm for 17 h. After 17 h, the arrays were washed as described in the One-Color Microarray-Based Gene Expression Analysis Low input Quick Amp Labelling protocol from Agilent. The arrays were scanned using the DNA microarray scanner with SureScan High-Resolution Technology from Agilent Technologies. Agilent Scan Control with resolution of 5 µm, 16 bits, and PMT of 100%. Feature extraction was performed using protocol 10.7.3.1 (version 10.7) for 1-color gene expression.
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