Log In Sign up
The largest database of trusted experimental protocols

Hm csf

Manufactured by Merck Group
Visit Supplier
Sourced in United States

HM-CSF is a lab equipment product manufactured by Merck Group. It is designed for the measurement and analysis of colony-stimulating factor (CSF) levels in various biological samples. The core function of HM-CSF is to provide researchers and scientists with a reliable and accurate tool for studying the role of CSF in different biological processes and disease states.

Automatically generated - may contain errors

Lab products found in correlation

Monocyte isolation kit 2, by Miltenyi Biotec (2 mentions) Human granulocyte macrophage colony stimulating factor hgm csf, by Merck Group (2 mentions) Hgm csf, by Merck Group (2 mentions) Facs canton 2, by BD (2 mentions) Human macrophage colony stimulating factor, by Merck Group (2 mentions) Lipopolysaccharides (lps), by Merck Group (2 mentions) Monocyte attachment medium, by PromoCell (2 mentions) Automacs pro separator, by Miltenyi Biotec (1 mentions)

3 protocols using hm csf

1

Monocyte Isolation and Macrophage Differentiation

Check if the same lab product or an alternative is used in the 5 most similar protocols
Monocytes were purified from PBMCs by negative selection (Monocyte Isolation Kit II; Miltenyi Biotec). Monocytes were suspended in monocyte attachment medium (PromoCell) and seeded at a density of 150,000/cm2 for 2 hours. Monocytes were co-stained with APC-Cy7-conjugated anti-CD11b, PE-Cy7-conjugated CD11c, Fitc conjugated anti-CD14, APC-conjugated CD16 or relative isotype control and then were analyzed using a FACS Canton II (BD Biosciences).
Primary human monocytes were differentiated into M1 macrophages using 20 ng/mL human Granulocyte-macrophage colony-stimulating factor (hGM-CSF, Sigma) or into M2 macrophages using 100 ng/mL human macrophage colony-stimulating factor (hM-CSF, Sigma) for 10 days. hGM-CSF differentiated M1 macrophages were stimulated with or without LPS (Sigma) 100 ng/mL for 6 hours.
Zhou Q., Wang H., Schwartz D.M., Stoffels M., Park Y.H., Zhang Y., Yang D., Demirkaya E., Takeuchi M., Tsai W.L., Lyons J.J., Yu X., Ouyang C., Chen C., Chin D.T., Zaal K., Chandrasekharappa S.C., Hanson E.P., Yu Z., Mullikin J.C., Hasni S.A., Wertz I., Ombrello A.K., Stone D.L., Hoffmann P., Jones A., Barham B.K., Leavis H.L., van Royen-Kerkof A., Sibley C., Batu E.D., Gül A., Siegel R.M., Boehm M., Milner J.D., Ozen S., Gadina M., Chae J., Laxer R.M., Kastner D.L, & Aksentijevich I. (2015). Loss-of-function mutations in TNFAIP3 leading to A20 haploinsufficiency cause an early onset autoinflammatory syndrome. Nature genetics, 48(1), 67-73.
+ Open protocol
+ Expand
2

Monocyte Isolation and Macrophage Differentiation

Check if the same lab product or an alternative is used in the 5 most similar protocols
Monocytes were purified from PBMCs by negative selection (Monocyte Isolation Kit II; Miltenyi Biotec). Monocytes were suspended in monocyte attachment medium (PromoCell) and seeded at a density of 150,000/cm2 for 2 hours. Monocytes were co-stained with APC-Cy7-conjugated anti-CD11b, PE-Cy7-conjugated CD11c, Fitc conjugated anti-CD14, APC-conjugated CD16 or relative isotype control and then were analyzed using a FACS Canton II (BD Biosciences).
Primary human monocytes were differentiated into M1 macrophages using 20 ng/mL human Granulocyte-macrophage colony-stimulating factor (hGM-CSF, Sigma) or into M2 macrophages using 100 ng/mL human macrophage colony-stimulating factor (hM-CSF, Sigma) for 10 days. hGM-CSF differentiated M1 macrophages were stimulated with or without LPS (Sigma) 100 ng/mL for 6 hours.
Zhou Q., Wang H., Schwartz D.M., Stoffels M., Park Y.H., Zhang Y., Yang D., Demirkaya E., Takeuchi M., Tsai W.L., Lyons J.J., Yu X., Ouyang C., Chen C., Chin D.T., Zaal K., Chandrasekharappa S.C., Hanson E.P., Yu Z., Mullikin J.C., Hasni S.A., Wertz I., Ombrello A.K., Stone D.L., Hoffmann P., Jones A., Barham B.K., Leavis H.L., van Royen-Kerkof A., Sibley C., Batu E.D., Gül A., Siegel R.M., Boehm M., Milner J.D., Ozen S., Gadina M., Chae J., Laxer R.M., Kastner D.L, & Aksentijevich I. (2015). Loss-of-function mutations in TNFAIP3 leading to A20 haploinsufficiency cause an early onset autoinflammatory syndrome. Nature genetics, 48(1), 67-73.
+ Open protocol
+ Expand
3

Differentiation of Macrophages from PBMCs

Check if the same lab product or an alternative is used in the 5 most similar protocols
Isolation of the peripheral blood mononuclear cells (PBMCs) from human peripheral blood donated by healthy volunteers was conducted with Human PBMC Separation Kit (Solarbio, Beijing, China). Thereafter, the CD14+ PBMCs were strained from human peripheral blood with auto-MACS Pro Separator (Miltenyi Biotec GmbH, Germany). To stimulate the macrophage differentiation, CD14+ PBMCs were treated with human M-CSF (hM-CSF) (25 ng/mL, Sigma, SRP3110, USA) for 6 days to obtain M0-Mφs. The M0-Mφs were continued to be cultured in 50% neuroblastoma-conditioned medium (NBCM) for 2 days to obtain the TAMs-Mφs.
Deng L., Huang S., Chen B., Tang Y., Huang F., Li D, & Tang D. (2020). Tumor-Linked Macrophages Promote HCC Development by Mediating the CCAT1/Let-7b/HMGA2 Signaling Pathway. OncoTargets and therapy, 13, 12829-12843.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!