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Metal grids

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Metal grids are used as sample holders in electron microscopy. They provide a stable platform to mount and support specimens for analysis under the electron beam.

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Lab products found in correlation

Tecnai g2 twin transmission electron microscope, by Thermo Fisher Scientific (2 mentions) Whatman filter paper, by Cytiva (2 mentions)

2 protocols using metal grids

1

Transmission Electron Microscopy Sample Preparation

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
As previously published with some modifications (Manning and Kuehn, 2011 (link)), to prepare samples for TEM imaging, metal grids (Electron Microscopy Sciences; 300 mesh copper, formvar/carbon) were cleaned using the Pelco EasiGlow Machine Vacuum and then 10 μL of sample was added. Samples were pipetted onto the grid and let set for 1 min before wicking away excess with a Whatman filter paper. The samples were stained by applying 10 μL of 2% uranyl acetate, incubated 1.5 min, and excess uranyl acetate removed by wicking with Whatman filter paper. Grids were stored in Petri dishes at room temperature. Samples were imaged using a FEI Tecnai G2 Twin Transmission Electron Microscope with a spot size of 2 at a voltage of 120kV.
McMillan H.M., Zebell S.G., Ristaino J.B., Dong X, & Kuehn M.J. (2021). Protective plant immune responses are elicited by bacterial outer membrane vesicles. Cell reports, 34(3), 108645.
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2

Transmission Electron Microscopy Sample Preparation

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
As previously published with some modifications (Manning and Kuehn, 2011 (link)), to prepare samples for TEM imaging, metal grids (Electron Microscopy Sciences; 300 mesh copper, formvar/carbon) were cleaned using the Pelco EasiGlow Machine Vacuum and then 10 μL of sample was added. Samples were pipetted onto the grid and let set for 1 min before wicking away excess with a Whatman filter paper. The samples were stained by applying 10 μL of 2% uranyl acetate, incubated 1.5 min, and excess uranyl acetate removed by wicking with Whatman filter paper. Grids were stored in Petri dishes at room temperature. Samples were imaged using a FEI Tecnai G2 Twin Transmission Electron Microscope with a spot size of 2 at a voltage of 120kV.
McMillan H.M., Zebell S.G., Ristaino J.B., Dong X, & Kuehn M.J. (2021). Protective plant immune responses are elicited by bacterial outer membrane vesicles. Cell reports, 34(3), 108645.
+ Open protocol
+ Expand

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