Total protein of 8−10 μg was denatured and subjected to SDS-PAGE, transferred to polyvinylidene fluoride (PVDF) membrane, and probed with antibodies, followed by HRP-conjugated secondary antibodies. Specific proteins were detected by ECL Western Blotting Detection Reagents (GE Healthcare Biosciences). Bovine serum albumin antibody was purchased from Thermoscientific (cat. # A11133). β-actin antibody was obtained from Sigma. The antibody against Nestin was purchased from Millipore (Temecula, CA). Antibodies against Glyceraldehyde 3-phosphate dehydrogenase (GAPDH), protein kinase B (AKT), extracellular signal-regulated kinase (ERK) and Focal Adhesion Kinase (FAK) were obtained from Cell Signaling Technology (Danvers, MA). For quantification of protein levels, densitometric analysis was carried out using ImageJ software (National Institutes of Health, Baltimore, MD).
Antibody against nestin
Manufactured by Merck Group
Sourced in Canada
Antibody against Nestin is a laboratory reagent used for the detection and identification of the Nestin protein. Nestin is an intermediate filament protein that is commonly used as a marker for neural stem and progenitor cells. This antibody can be utilized in various research applications, such as immunohistochemistry, Western blotting, and flow cytometry, to study the expression and distribution of Nestin in biological samples.
Automatically generated - may contain errors
Lab products found in correlation
Ecl western blotting detection reagent, by GE Healthcare (2 mentions)
Bovine serum albumin antibody, by Thermo Fisher Scientific (2 mentions)
Antibodies against glyceraldehyde 3 phosphate dehydrogenase gapdh, by Cell Signaling Technology (2 mentions)
β actin antibody, by Merck Group (2 mentions)
Focal adhesion kinase, by Cell Signaling Technology (2 mentions)
2 protocols using antibody against nestin
1
Western Blot Protein Detection
2
Quantitative Western Blot Analysis
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