Il 17a alexafluor647

Manufactured by Thermo Fisher Scientific

The IL-17A-AlexaFluor647 is a fluorescently labeled antibody that specifically binds to the IL-17A protein. It is designed for use in flow cytometry and other immunodetection applications.

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IL-17A (306 citations) AlexaFluor647 anti-IL-17A (2 citations)

2 protocols using il 17a alexafluor647

Comprehensive Immune Cell Profiling

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CFSE labeling of cells was performed according to manufacturer's instructions (Cayman Chemicals). After fixation, permeabilization and blockade of nonspecific binding (buffers from eBioscience) cells were stained with appropriate combinations of the following antibodies: CD4-FITC (eBioscience), CD4-PerCP (BD), CD25-Pacific Blue (Biolegend) Foxp3-PE (eBioscience), Helios-allophycocyanin (Biolegend), Nrp-1-allophycocyanin (R&D Systems), ROR-γt-PE (eBioscience), and IL-17A-AlexaFluor647 (eBioscience). Cells were incubated with 25 ng/mL PMA, 250 ng/mL ionomycin, and 1 μL/mL brefeldin A (BD) for 5 h prior to intracellular cytokine staining. Samples were acquired on the BD LSR II and analyzed with FlowJo (Tree Star) or FCS express (De Novo) software.

Whibley N., MacCallum D.M., Vickers M.A., Zafreen S., Waldmann H., Hori S., Gaffen S.L., Gow N.A., Barker R.N, & Hall A.M. (2014). Expansion of Foxp3+ T-cell populations by Candida albicans enhances both Th17-cell responses and fungal dissemination after intravenous challenge. European Journal of Immunology, 44(4), 1069-1083.

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Murine Myeloid Dendritic Cell Immunophenotyping

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Single lung or LN cell suspensions (1×10⁶ cells/100 μl) were incubated in FACS buffer (PBS, 1% FBS, 0.05% NaN₃) containing CD16/32 Fc-blocking antibody (2.4G2, BD Biosciences, San Jose, CA) for 30 minutes at 4°C and then stained with fluorescently-labeled antibodies for 30 minutes at 4°C. Cells were washed repeatedly in FACS buffer, fixed in 2% PFA for 20 minutes at room temperature, washed and resuspended in FACS buffer for flow cytometry analysis. Data was acquired using the LSR II flow cytometer (BD Biosciences) and analyzed using FACSDiVa (BD Biosciences) and FlowJo software (TreeStar, Inc. Ashland, OR). The following mouse-specific monoclonal antibodies were purchased from eBioscience with the clone indicated in parentheses: CD11c-Alexa Fluor 647 (HL3), CD11b-PE-Cy7 (M1/70), Gr1-APC-eFluor780 (RB6-8C5), CD317-Alexa Fluor 488 (eBio927), CD80-PE (16-10A1), CD86-PE (GL1), PD-L1-PE (MIH5), B7-DC-PE (TY25), CD4-PE-Cy7 (RM4-5), IL-13-PE (eBio13A) and IL-17A-Alexa Fluor 647 (eBio17b7). Myeloid DCs were defined as CD11c⁺CD11b⁺Gr1⁻CD317⁻.

Yang L., Lewkowich I., Apsley K., Fritz J.M., Wills-Karp M, & Weaver T.E. (2015). Haploinsufficiency for Stard7 is Associated With Enhanced Allergic Responses in Lung and Skin. Journal of immunology (Baltimore, Md. : 1950), 194(12), 5635-5643.

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