Ebselen

Manufactured by Bio-Techne

Sourced in United Kingdom, Germany

Ebselen is a small organic compound with the chemical formula C13H9NOSe. It functions as a selenium-containing organic compound that can mimic the activity of the antioxidant enzyme glutathione peroxidase.

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Lab products found in correlation

3 protocols using ebselen

Mitochondrial Stress Response Modulators

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Compounds were used at the following concentrations unless otherwise noted: 50 μM AZD3988 (Tocris), 30 μM A922500 (Stratech), 50 μM AZD7687 (Stratech), 70 μM T863 (Sigma), 1 μM Oligomycin (Sigma), 0.5 μM FCCP (Sigma), 1 μM Antimycin-A (Sigma), 1 μM Rotenone (Sigma), 50 μM PF-06424439 (Sigma), 5 μM Ebselen (Tocris), 1 mM Tempol, 200 μM Paraquat (Sigma), Menadione (Sigma), 100 μM Etomoxir (Sigma), 2 μM Ferrostatin-1 (Sigma), 5 μM ML385 (Selleckchem), and 1 μM ATN-224 (Cayman Chemical). Antibodies against DGAT1 (ab54037) and 4-Hydroxynonenal (ab46545) were purchased from abcam. Antibodies against Vinculin (66305-1-Ig), Beta-Tubulin (10094-1-AP), PINK1 (23274-1-AP), Parkin (14060-1-AP), SOD1 (10269-1-AP), SOD2 (24127-1-AP), SESN2 (10795-1-AP) and GAPDH (60004-1-Ig) were purchased from Proteintech. Antibodies against phospho-AMPK (50081), phospho-RAPTOR (2083), Caspase-3 (9662), phospho-ERK (4370) and GFP (2956) were purchased from Cell Signalling. The antibody against gamma-tubulin (T5326) was purchased from Sigma. The antibody against ERK2 (C-14) was from Santa Cruz Biotechnology.

Wilcock D.J., Badrock A.P., Wong C.W., Owen R., Guerin M., Southam A.D., Johnston H., Telfer B.A., Fullwood P., Watson J., Ferguson H., Ferguson J., Lloyd G.R., Jankevics A., Dunn W.B., Wellbrock C., Lorigan P., Ceol C., Francavilla C., Smith M.P, & Hurlstone A.F. (2022). Oxidative stress from DGAT1 oncoprotein inhibition in melanoma suppresses tumor growth when ROS defenses are also breached. Cell Reports, 39(12), 110995.

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Ebselen Modulates SHIP2 Phosphatase Activity

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Recombinant His-tagged human SHIP2 and SHIP1 phosphatase domains were produced as previously described 26 (link). SHIP2 was immunoprecipitated as in 17 (link) from lysates of myotubes and hepatoma cells treated with ebselen (MilliporeSigma) at indicated concentrations and times or from lysates of tissues of mice treated with ebselen (Tocris Bioscience, Bristol, UK or Cayman Chemicals, MI, USA) for 12 days or 10 weeks and mice treated with metformin (MilliporeSigma) for 12 days.

Polianskyte-Prause Z., Tolvanen T.A., Lindfors S., Kon K., Hautala L.C., Wang H., Wada T., Tsuneki H., Sasaoka T, & Lehtonen S. (2022). Ebselen enhances insulin sensitivity and decreases oxidative stress by inhibiting SHIP2 and protects from inflammation in diabetic mice. International Journal of Biological Sciences, 18(5), 1852-1864.

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Analysis of PAF-induced Neutrophil Responses

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PAF-induced effects on neutrophils were analyzed in the presence of the subsequently listed pharmacological modulators (their proposed targets are given in brackets): Amiloride [200 μM, 10 min, Na⁺-channels (15 (link))], BIX [5 μM, 30 min, sodium-proton exchanger 1 (NHE1) (38 (link)); Tocris, Wiesbaden, Germany], 5-Nitro-2-(3-phenylpropylamino)benzoic acid [NPPB, 100 μM, 10 min, Cl⁻ channels (39 (link))], and ebselen [10 nM, 30 min, glutathione peroxidase and peroxiredoxin enzyme mimetic and NOX2 inhibitor (40 (link), 41 (link)); Tocris]. Following pre-incubation, samples were divided to obtain paired results and treated as control or stimulated with PAF.

Hug S., Bernhard S., Stratmann A.E., Erber M., Wohlgemuth L., Knapp C.L., Bauer J.M., Vidoni L., Fauler M., Föhr K.J., Radermacher P., Hoffmann A., Huber-Lang M, & Messerer D.A. (2021). Activation of Neutrophil Granulocytes by Platelet-Activating Factor Is Impaired During Experimental Sepsis. Frontiers in Immunology, 12, 642867.

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