Anti gr

Proteins were separated on SDS polyacrylamide gels were transferred to Pure Nitrocellulose Blotting membrane (Pall). Blots were blocked with 1.2 g milk in 100 ml buffer containing sodium chloride (0.88 g) and 0.1% Tween 20. Membrane was incubated with primary antibody for overnight at 4 °C. Secondary antibody was added to membrane for 1 h at room temperature. Antibodies used in this study: anti-GR (Proteintech), S. cerevisiae anti-Hog1 was used to detected total Sty1 (Santa Cruz), human p-p38 antibody (Cell Signalling Technologies) was used to detected p-Sty1, S. cerevisiae anti- Rps3 was used in order to identify the ribosomal subunit^{43 (link)}. Immune complexes were visualized with the Licor Odyssey system.

Total cell extracts or nuclear extracts were separated by SDS-PAGE and transferred to PVDF membranes. The following antibodies were used for immunoblot analysis: anti-HK2, anti-PFKM, anti-LDHa1, anti-PDK2, anti-PDHa1, anti-ATP5B, anti-IDH2, anti-HIF1α, anti-MnSOD, anti-Cu/ZnSOD, anti-Catalase, anti-GR, anti-GRX1, anti-GPX1, anti-PRX3 and anti-TRX2 antibodies and secondary antibody were purchased from Proteintech. anti-MnSOD (acetyl K68) antibody was purchased from Abcam. Anti-β-actin, anti-ComplexV, anti-Lc3B, anti-MFN1, and anti-Fis1 antibodies were from Cell Signaling Technology. Protein expression is visualized on Tanon-5200 Chemi-luminescent Imaging System (Tanon Science Technology).