Dimethyl k9 histone h3

Manufactured by Merck Group

Sourced in United States

Dimethyl K9 histone H3 is a type of laboratory equipment used for the detection and analysis of histone modifications. It is a specific antibody that recognizes the dimethylation of lysine 9 on histone H3, which is an important epigenetic mark involved in gene regulation and chromatin structure.

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Lab products found in correlation

Magna chip g chromatin immunoprecipitation kit, by Merck Group (2 mentions) Trimethyl k9 histone h3, by Merck Group (2 mentions) Trimethyl k27 histone h3, by Abcam (2 mentions)

Spelling variants of this product

Histone H3 (77 citations) Histones (7 citations)

2 protocols using dimethyl k9 histone h3

ChIP Analysis of SULF1 Promoter

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Chromatin immunoprecipitation (ChIP) was performed from nuclear extracts of SNU182, SNU449 and Hep3B cells using the Magna ChIP™ G Chromatin Immunoprecipitation Kit (Millipore, Billerica, Mass., USA). Antibodies against dimethyl K9 histone H3 (Millipore), trimethyl K9 histone H3 (Millipore), acetyl (K9, K14) histone H3 (Millipore) and trimethyl K27 histone H3 (Abcam, Cambridge, Mass., USA) and a control antibody were used. Specific primers for 170-bp [5′-GAGATGGCCCTTCCATAGACG-3′ (forward; −205 of TSS) and 5′-GAGAAGTGGGAGGTGCTACAG-3′ (reverse; −36 of TSS)] and 205-bp [5′-GCCACGATCTAATCAAAGAAAGG-3′ (forward; −348 of TSS) and 5′-CTCAGTGGGATGCACAGAAAC-3′ (reverse; −144 of TSS)] regions of the SULF1 promoter were utilized for PCR amplification. PCR products were separated on a 2% agarose gel.

Shire A., Lomberk G., Lai J.P., Zou H., Tsuchiya N., Aderca I., Moser C.D., Gulaid K.H., Oseini A., Hu C., Warsame O., Jenkins R.B, & Roberts L.R. (2015). Restoration of Epigenetically Silenced Sulfatase 1 Expression by 5-Aza-2′-Deoxycytidine Sensitizes Hepatocellular Carcinoma Cells to Chemotherapy-Induced Apoptosis. Medical Epigenetics, 3(1), 1-18.

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Chromatin Immunoprecipitation of Histone Modifications

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ChIP was performed from nuclear extracts of SNU182, SNU449 and Hep3B cells using the Magna ChIP™ G Chromatin Immunoprecipitation Kit (Millipore; Billerica, MA). Antibodies against di-methyl K9 histone H3 (Millipore), tri-methyl K9 histone H3 (Millipore), acetyl (K9, K14) histone H3 (Millipore), tri-methyl K27 histone H3 (Abcam, Cambridge, MA) and a control antibody were used. Specific primers for 170 bp [5’-GAGATGGCCCTTCCATAGACG-3’ (forward, -205 of TSS) and 5’-GAGAAGTGGGAGGTGCTACAG-3’ (reverse; -36 of TSS)] and 205bp [5’-GCCACGATCTAATCAAAGAAAGG-3’ (forward; -348 of TSS) and 5’-CTCAGTGGGATGCACAGAAAC-3’ (reverse; -144 of TSS)] regions of the SULF1 promoter were utilized for PCR amplification. PCR products were separated on a 2% agarose gel.

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