The human lung adenocarcinoma cell lines A549, HCC827, NCI-1395, and NCI-H1650 were obtained from the American Type Culture Collection (ATCC). PC9 and NCI-H1975 cells were provided by Dr Fan (Department of Thoracic Surgery, Fourth Military Medical University, Xi’an, China). All cells were grown in Roswell Park Memorial Institute (RPMI) 1640 complete medium (Gibco, USA) supplemented with 10% fetal bovine serum (FBS; Gibco, USA), 100 U/mL penicillin (Thermo Fisher Scientific, MA, USA), and 100 ug/mL streptomycin (Thermo Fisher Scientific, MA, USA). All cells were cultured in a humidified atmosphere with 5% CO2 at 37°C. Cell lines were routinely authenticated, and mycoplasma tested. Ribociclib was purchased from Selleck, China, and pemetrexed was from Med Chem Express, China. Drugs were dissolved in dimethyl sulfoxide (DMSO, Sigma, USA) and stored at -20°C.
Pemetrexed
Manufactured by MedChemExpress
Sourced in China
Pemetrexed is a chemical compound used as a laboratory reagent. It is a folate antimetabolite that inhibits multiple enzymes involved in folate metabolism, resulting in the disruption of cellular processes. Pemetrexed is commonly used in research applications to study cell proliferation and cancer-related pathways.
Automatically generated - may contain errors
Lab products found in correlation
Penicillin, by Thermo Fisher Scientific (2 mentions)
Streptomycin, by Thermo Fisher Scientific (2 mentions)
1640 complete medium, by Thermo Fisher Scientific (1 mentions)
Ribociclib, by Selleck Chemicals (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (1 mentions)
Hcc827, by American Type Culture Collection (1 mentions)
Nci h1650, by American Type Culture Collection (1 mentions)
Ofloxacin, by MedChemExpress (1 mentions)
Lamotrigine, by MedChemExpress (1 mentions)
Digoxin, by MedChemExpress (1 mentions)
Atenolol, by MedChemExpress (1 mentions)
Carbamazepine, by MedChemExpress (1 mentions)
Propranolol, by MedChemExpress (1 mentions)
Diltiazem, by MedChemExpress (1 mentions)
Ganciclovir, by MedChemExpress (1 mentions)
Quinidine, by MedChemExpress (1 mentions)
Citalopram, by MedChemExpress (1 mentions)
Sumatriptan, by Tokyo Chemical Industry (1 mentions)
Acetaminophen, by Merck Group (1 mentions)
2 protocols using pemetrexed
1
Lung Adenocarcinoma Cell Line Cultivation
2
Physicochemical Characterization of Diverse Compounds
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In all, 18 compounds with different physiochemical properties were selected for this study. Antipyrine, ganciclovir, ofloxacin, 4‐aminoAntipyrine, lamotrigine, theophylline, citalopram, digoxin, quinidine, pemetrexed, atenolol, carbamazepine, propranolol, and diltiazem were purchased from MedChemExpress (Monmouth Junction). Acetaminophen was purchased from Sigma‐Aldrich. Fexofenadine and sumatriptan were purchased from Tokyo Chemical Industry. Cimetidine was purchased from National Institute for the Control of Pharmaceutical and Biological Products. All the other chemicals were of the HPLC grade or better.
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