Colistin

Manufactured by Mast Group

Sourced in United Kingdom

Colistin is a type of antibiotic used for laboratory analysis and research purposes. It is a polymyxin class antibiotic that acts by disrupting the cell membrane of certain bacteria. Colistin is often used in research settings to study bacterial susceptibility and resistance.

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3 protocols using colistin

Antibiotic Susceptibility Testing of Enterobacteriaceae

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AST was performed using disk diffusion method (Kirby–Bauer) on Mueller–Hinton agar (Merck) plates according to the Clinical and Laboratory Standards Institute (CLSI) Guidelines.22 The tested antibiotic disks were colistin (10 μg), ciprofloxacin (5 μg), tetracycline (30 μg), imipenem (10 μg), ceftazidime (30 μg), azithromycin (15 μg) and amikacin (30 μg) (MAST Co., UK). The phenotype of Enterobacteriaceae was defined as MDR according to the International Expert proposal for Interim Standards Guidelines.6 (link)

Moosavian M, & Emam N. (2019). The first report of emerging mobilized colistin-resistance (mcr) genes and ERIC-PCR typing in Escherichia coli and Klebsiella pneumoniae clinical isolates in southwest Iran. Infection and Drug Resistance, 12, 1001-1010.

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Antibiotic Resistance Profiling of E. coli

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DST was performed using the disk diffusion method (Kirby-Bauer) on Mueller-Hinton agar (Merck, Germany) plates according to the Clinical and Laboratory Standards Institute (CLSI) guidelines [25 ]. The tested antibiotic disks were: colistin (COL, 10μg), ciprofloxacin (CIP, 5μg), tetracycline (T, 30μg), imipenem (IMI, 10μg), ceftazidime (CAZ, 30μg), azithromycin (AT, 15μg), and amikacin (AK, 30μg) (MAST Co., UK). The phenotype of E.coli was defined as MDR according to the International Expert proposal for Interim Standards Guidelines [26 (link)]. Minimum Inhibitory Concentration (MIC) of colistin (Sigma-Aldrich, USA) was determined using the E-test strips (Mast, UK) method according to CLSI guidelines.

Moosavian M., Emam N., Pletzer D, & Savari M. (2020). Rough-type and loss of the LPS due to lpx genes deletions are associated with colistin resistance in multidrug-resistant clinical Escherichia coli isolates not harbouring mcr genes. PLoS ONE, 15(5), e0233518.

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Antimicrobial Susceptibility Testing for Bacterial Isolates

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Antimicrobial susceptibility testing for the bacterial isolates was carried out using the Kirby-Bauer method, as recommended by the Clinical and Laboratory Standards Institute (CLSI). 15 The following antibiotics were tested: imipenem (10 μg), meropenem (10 μg), doripenem (10 μg), ceftazidime (30 μg), cefepime (30 μg), piperacillin (100 μg), piperacillin/tazobactam (100/10 μg), gentamicin (10 μg), amikacin (30 μg), tobramycin (10 μg), ciprofloxacin (5 μg), aztreonam (30 μg), polymyxin B (300 units), and colistin (10 μg) (Mast Group Ltd, UK). The minimum inhibitory concentrations (MICs) of carbapenems (imipenem [IMI], meropenem [MRP], and doripenem [DOR]) were obtained by an E-test (Liofilchem, Italy), as described in the manufacturer's instructions. Carbapenem resistance was determined based on the MIC breakpoints. When an isolate was resistant to three carbapenems (imipenem, meropenem, and doripenem), that isolate was considered high-level carbapenem resistant. If an isolate was resistant to three or more classes of antimicrobial agents (i.e., penicillins/cephalosporins, carbapenems, aminoglycosides, and fluoroquinolones), that isolate was considered multidrug resistant (MDR). In accordance with the CLSI guidelines, P. aeruginosa ATCC 27853 and Escherichia coli ATCC 25922 were used as control strains in all susceptibility assays.

Rostami S., Farajzadeh Sheikh A., Shoja S., Farahani A., Tabatabaiefar M.A., Jolodar A, & Sheikhi R. (2018). Investigating of four main carbapenem-resistance mechanisms in high-level carbapenem resistant Pseudomonas aeruginosa isolated from burn patients. Journal of the Chinese Medical Association : JCMA, 81(2).

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