The CCDC170 orf (RefSeq accession NM_025059) was obtained from OriGene Technologies in the pCMV6-AC-GFP C-terminal turboGFP fusion vector. The CCDC170 orf was transferred to the pCMV6-AN-mGFP N-terminal monomeric GFP fusion vector using the OriGene Precision Shuttle cloning sites Sgf I and Mlu I. The CCDC170 wild-type gene was subsequently subcloned into a pTRE-Tight (Clontech) at Kpn I and Not I sites. Fragments of CCDC170 1-48, 1-405, 1-591, 1-649, 1-689, 355-591, 355-689, 355-715, 593-715 were generated in the N-terminal GFP CCDC170 fusions by either gene synthesis (GenScript) or using the Agilent QuickChange II site-directed mutagenesis kit. Non-tag wild-type CCDC170 was also subcloned in the pRetroX-IRES-ZsGreen1 vector at the Not I site.
Jiang P., Li Y., Poleshko A., Medvedeva V., Baulina N., Zhang Y., Zhou Y., Slater C.M., Pellegrin T., Wasserman J., Lindy M., Efimov A., Daly M., Katz R.A, & Chen X. (2017). The Protein Encoded by the CCDC170 Breast Cancer Gene Functions to Organize the Golgi-Microtubule Network. EBioMedicine, 22, 28-43.
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