One series of 20-μm sections mounted on RNase-free Superfrost slides was subjected to Nissl staining with 0.1% thionin (Sigma-Aldrich), dehydrated, immersed in xylene and cover-slipped with Roti-Histokitt II mounting medium (Roth). The HVC areas (typically 8–10 slices) were measured with a Leica DM6000 B microscope connected to a computer-based image-analysis system (IMAtec). All brains were coded such that the observers were blind toward any additional information about the sections they measured during the delineations. The volumes were derived from the summed area measurements multiplied by the section thickness and the intersection distance.
Roti histokitt 2 mounting medium
Manufactured by Carl Roth
Roti-Histokitt II is a mounting medium designed for use in histological and microscopic applications. It is intended to be used for mounting and preserving tissue samples on microscope slides.
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2 protocols using roti histokitt 2 mounting medium
1
Nissl Staining for Brain Volume Analysis
2
Nissl Staining and HVC Volume Quantification
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One series of 20-μm sections mounted on RNase-free Superfrost slides was subjected to Nissl staining with 0.1% thionin (Sigma-Aldrich), dehydrated, immersed in xylene and cover-slipped with Roti-Histokitt II mounting medium (Roth). The HVC areas (typically 8-10 slices) were measured with a Leica DM6000 B microscope connected to a computerbased image-analysis system (IMAtec). All brains were coded such that the observers were blind towards any additional information about the sections they measured during the delineations. The volumes were derived from the summed area measurements multiplied by the section thickness and the intersection distance.
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