Exposure cassete k

Manufactured by Bio-Rad

Sourced in Spain

The Exposure Cassette-K is a laboratory equipment used for the analysis of protein samples in Western blot experiments. It provides a controlled environment for exposing photographic film or imaging plates to the chemiluminescent or radioactive signals generated during the blotting process. The cassette ensures consistent exposure conditions, enabling reliable and reproducible results.

Automatically generated - may contain errors

Lab products found in correlation

Molecular imager fx image acquisition system, by Bio-Rad (2 mentions) Quantity one image analysis software, by Bio-Rad (2 mentions)

2 protocols using exposure cassete k

Radioactive Lipid Separation and Quantification

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Another aliquot of 0.1 mg of TL extract from radioactive samples was also applied to HPTLC plates to determine the esterification of [1-14 C]FA into LC. Lipid classes were separated as mention on 2.5. Developed HPTLC plates were placed for 1 week in closed exposure cassettes (Exposure Cassete-K, BioRad, Madrid, Spain) in contact with a radioactive-sensitive phosphorus screen (Imagen Screen-K, Biorad). The screens were then scanned with the Molecular Imager FX image acquisition system (BioRad), and the bands were quantified in percentage by Quantity One image analysis software (BioRad).

Reis D.B., Acosta N.G., Almansa E., Garrido D., Andrade J.P., Sykes A.V, & Rodríguez C. (2019). Effect of Artemia inherent fatty acid metabolism on the bioavailability of essential fatty acids for Octopus vulgaris paralarvae development. Aquaculture (Amsterdam, Netherlands), 500.

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Esterification Pattern of [1-14C]ARA in Hatchlings

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An aliquot of 0.1 mg of hatchlings TL was taken for determine the esterification pattern of [1-14 C]ARA into the different LC. Lipid classes were separated by one-dimensional double-development HPTLC as previously described 2.4. Developed HPTLC plates were placed for 2 weeks in closed exposure cassettes (Exposure Cassete-K, BioRad, Madrid, Spain) in contact with a radioactive-sensitive phosphorus screen (Imagen Screen-K, Biorad, Madrid, Spain). The screens were then scanned with Molecular Imager FX image acquisition system (BioRad, Madrid, Spain), and the bands were quantified using the Quantity One image analysis software (BioRad, Madrid, Spain). Identification of labelled bands was confirmed by radiolabelled standards simultaneously run on the same plate (Rodríguez et al., 2002) . andL-∝-1-palmitoyl-2-[1-14 C

Reis D.B., Acosta N.G., Almansa E., Tocher D.R., Andrade J.P., Sykes A.V, & Rodríguez C. (2016). Composition and metabolism of phospholipids in Octopus vulgaris and Sepia officinalis hatchlings. Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology, 200.

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