Uc30 microscope digital camera

The test was performed in glass blocks with hemispherical cavity of 32 mm in diameter (Karl Hecht Staining Blocks Molded Glass 2020, Lab Unlimited). Only adult and fully active (displaying coordinated movements of the body and legs) specimens with a body length of about 200 µm and cleaned of debris were collected 16 h before the test beginning and kept in the glass block in 3 ml of the culture medium. To check the impact of KCN, AA and BHAM, groups of 20 fully active specimens were transferred into 1 ml of the culture medium in new glass blocks. Then they were treated with combinations of KCN, BHAM, AA and methanol (used as a solvent for AA and BHAM) for 2 h at 18 o C and observed in a few time points (i.e. 30 min, 45 min and 2 h). The applied concentrations of these inhibitors were established experimentally to obtain saturated effect on the oxygen uptake rate inhibition (see Measurements of H. exemplaris respiration) and were as follows: 1 mM KCN, 3 mM BHAM and 280 µg/ml AA. The test was repeated two times. The specimens activity before and after the treatment was monitored under Olympus SZ61 stereo microscope connected to Olympus UC30 microscope digital camera. Images and short video films were obtained using Olympus CellSens Standard Software.

The test was performed in glass blocks with hemispherical cavity of 32 mm in diameter (Karl Hecht Staining Blocks Molded Glass 2020, Lab Unlimited). Only adult and fully active (displaying coordinated movements of the body and legs) specimens with a body length of about 200 µm and cleaned of debris were collected 16 h before the test beginning and kept in the glass block in 3 ml of the culture medium.
To check the impact of KCN, AA and BHAM, groups of 20 fully active specimens were transferred into 1 ml of the culture medium in new glass blocks. Then they were treated with combinations of KCN, BHAM, AA and methanol (used as a solvent for AA and BHAM) for 2 h at 18 o C and observed in a few time points (i.e. 30 min, 45 min and 2 h). The applied concentrations of these inhibitors were established experimentally to obtain saturated effect on the oxygen uptake rate inhibition (see Measurements of H. exemplaris respiration) and were as follows: 1 mM KCN, 3 mM BHAM and 280 µg/ml AA. The test was repeated two times. The specimens activity before and after the treatment was monitored under Olympus SZ61 stereo microscope connected to Olympus UC30 microscope digital camera. Images and short video lms were obtained using Olympus CellSens Standard Software.