Ground steel maldi target

The matrix was prepared by dissolving 2,5-dihydroxybenzoïque (DHB) (2g.L-1) in 0.1% trifluoroacetic acid/acetonitrile (TFA/ACN) (30/70). Fraction collection was realized using Ground Steel MALDI target (Bruker Daltonics, Bremen, Germany). Mass spectra of the CE fractions were recorded using an Autoflex II MALDI-TOF (Bruker Daltonics, Bremen, Germany), operating in reflector mode and with FlexControl software. Positively charged ions were detected and sums of 2000 singleshot spectra were acquired automatically from each sample by using the AutoXecute software. For Topdown approach, sums of 15000 single-shot spectra were acquired automatically from each sample.
Data processing was performed with FlexAnalysis 3.0 provided by the mass spectrometer manufacturer. All spectra were calibrated according an external calibration using Protein calibration standard I (Bruker Daltonics, Bremen, Germany) for intact protein separation.

The matrix was prepared by dissolving 2,5-dihydroxybenzoic acid (DHB) (2g/L) in 0.1% trifluoroacetic acid/acetonitrile (TFA/ACN) (30/70)(v/v). Fraction collection was realized using Ground Steel MALDI target (Bruker Daltonics, Bremen, Germany). Mass spectra of the CZE fractions were recorded using an Autoflex II MALDI-TOF (Bruker Daltonics, Bremen, Germany), operating in reflector mode and with FlexControl software. Positively charged ions were detected and sums of 1500 single-shot spectra were acquired automatically from each sample by using the AutoXecute software.
Data processing was performed with FlexAnalysis 3.0 (Bruker Daltonics, Bremen, Germany). All spectra were calibrated by external calibration using Protein calibration standard I (Bruker Daltonics, Bremen, Germany) for intact protein separation.