Fetal bovine serum (FBS), 1640 medium, Dulbecco's Modified Eagle Medium (DMEM) medium, phosphate buffer saline (PBS), trypsin, streptomycin, and penicillin were purchased from KeyGen Biotech (Nanjing, China); Ficoll separation medium and dimethyl sulfoxide (DMSO) from Bio-swamp company (Wuhan, China); CD4, CD45RA, CXCR5, CCR6, CXCR3 and corresponding isotype control antibodies from Biolegend Company, USA; EPICS XL flow cytometer from BECKMAN COULT ER Company.
Phosphate buffer saline (pbs)
Manufactured by Keygen Biotech
Sourced in China
Phosphate buffer saline (PBS) is a commonly used buffer solution in biological and biochemical applications. It is an aqueous solution containing sodium phosphate, sodium chloride, and other salts, with a pH and osmolarity compatible with physiological conditions. PBS is used to maintain the stability and function of biological samples, cells, and molecules in various experimental and analytical procedures.
Automatically generated - may contain errors
Lab products found in correlation
Epics xl flow cytometer, by Beckman Coulter (1 mentions)
Trypsin, by Keygen Biotech (1 mentions)
Streptomycin, by Keygen Biotech (1 mentions)
Penicillin, by Keygen Biotech (1 mentions)
Facscalibur, by BD (1 mentions)
Cd105, by BD (1 mentions)
Mitomycin c, by Merck Group (1 mentions)
Dimethyl sulfoxide (dmso), by Thermo Fisher Scientific (1 mentions)
Uplc grade acetonitrile, by Merck Group (1 mentions)
Leucine enkephalin, by Waters Corporation (1 mentions)
Formic acid, by Merck Group (1 mentions)
Ginsenoside rb1, by Chengdu Must Bio-Technology (1 mentions)
Ginsenoside rd, by Chengdu Must Bio-Technology (1 mentions)
Ginsenoside re, by Chengdu Must Bio-Technology (1 mentions)
Ginsenoside rf, by Chengdu Must Bio-Technology (1 mentions)
Ginsenoside rg1, by Chengdu Must Bio-Technology (1 mentions)
Notoginsenoside r1, by Chengdu Must Bio-Technology (1 mentions)
Amyloid β protein fragment 25 35, by Merck Group (1 mentions)
Dulbecco s modified eagle medium dmem, by Keygen Biotech (1 mentions)
Pc12 cells, by Keygen Biotech (1 mentions)
5 protocols using phosphate buffer saline (pbs)
1
Isolation and Characterization of Human T Cell Subsets
2
Characterization of Human Adipose-Derived Stem Cells
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Flow cytometry was utilized to characterize the hADSCs. Cells at passage 3 were digested with 0.25% EDTA-trypsin and washed three times in phosphate buffer saline (PBS; Keygen, China) after centrifugation (1500 × g for 5 min). Afterward, 1 × 104 cells/tube were incubated in the dark at room temperature for 20 min with the following primary antibodies: CD44 (fluorescein isothiocyanate [FITC] conjugated), CD105 (allophycocyanin conjugated), CD45 (phycoerythrin [PE] conjugated), CD34 (PE conjugated) (all purchased from BD Bioscience, USA), and fluorescence line height-2 was set as blank. Then, the cells were washed again twice with PBS, centrifuged (1500 × g for 5 min) and resuspended in 200 μL of PBS. Finally, CD surface antigens were analyzed with a flow cytometer (BD FACSCalibur, USA).
3
Wound Healing Assay with Mitomycin C
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The cells were cultured in six-well plates at a density of 5 × 105 cells/mL. After the density reaching almost 100%, 1 μg/mL mitomycin C (Sigma-Aldrich) was added for 2 h, and then, a 10 μL sterile pipette tip was used to make a wound. The supernatant was then discarded after washing with phosphate buffer saline (KeyGEN BioTECH). Serum-free DMEM was added to the six-well plates, and the wound closure was measured every 24 h until the scratch was completely closed. The images were acquired using an inverted light microscope.
4
Ginsenosides and Alzheimer's Disease Treatment
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UPLC-grade acetonitrile was purchased from Merck (Darmstadt, Germany). Formic acid was purchased from Sigma-Aldrich (Mo, USA). Leucine enkephalin was obtained from Waters Corporation (Milford, MA, USA). Distilled water was obtained from Watson's Food & Beverage (Guangzhou, China).
The PC12 cells were purchased from Jiangsu KeyGEN BioTECH Corp., Ltd (Nanjing, China). Ginsenoside Rb1, ginsenoside Rb2, ginsenoside Rb3, ginsenoside Rc, ginsenoside Rd, ginsenoside Re, ginsenoside Rf, ginsenoside Rg1, notoginsenoside R1, 20(S)-notoginsenoside R2, notoginsenoside Fa, gypenosides A, and gypenosides XLIX were purchased from Chengdu Must Bio-Technology Co., Ltd. (Chengdu, China). The 20(S)-ginsenoside Rb2, galantamine HBr, and berberine were purchased from the National Institutes for Food and Drug Control (Beijing, China). The structure of these compounds is shown inFigure 2 . Dulbecco's modified Eagle medium (DMEM) and phosphate buffer saline (PBS) were purchased from Jiangsu KeyGEN BioTECH Corp., Ltd. (Nanjing, China). Fetal bovine serum (FBS), trypsin, and dimethyl sulfoxide (DMSO) were purchased from Gibco (Thermo Fisher Scientific, Inc., Waltham, MA, USA). Amyloid β-protein fragment 25–35 was purchased from Sigma-Aldrich (St. Louis, MO, USA). YZG was produced by Guangxi Wanshoutang Pharmaceutical Co., Ltd., and formulated by water into suspension.
The PC12 cells were purchased from Jiangsu KeyGEN BioTECH Corp., Ltd (Nanjing, China). Ginsenoside Rb1, ginsenoside Rb2, ginsenoside Rb3, ginsenoside Rc, ginsenoside Rd, ginsenoside Re, ginsenoside Rf, ginsenoside Rg1, notoginsenoside R1, 20(S)-notoginsenoside R2, notoginsenoside Fa, gypenosides A, and gypenosides XLIX were purchased from Chengdu Must Bio-Technology Co., Ltd. (Chengdu, China). The 20(S)-ginsenoside Rb2, galantamine HBr, and berberine were purchased from the National Institutes for Food and Drug Control (Beijing, China). The structure of these compounds is shown in
5
Isolation and Characterization of Anti-Cancer Triterpene Saponins from Actinidia chinensis Roots
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The roots of Actinidia chinensis were purchased from Wenzhou Hospital of Integrated Traditional Chinese and Western Medicine. The EERAC (ethanol extract from radix of Actinidia chinensis) was isolated in our laboratory according to extraction technology of effective anti-cancer active ingredient of Actinidia chinensis root from Institute of Chemistry, Chinese Academy of Sciences (Patent publication No.: CN1977869A). The main active chemical components of EERAC were triterpene saponins, which identified by Libermannn Burchard reaction and foam test. Phosphate buffer saline (PBS), Fetal bovine serum (FBS), and tryspin were purchased from KeyGEN Biotech (Nanjing, Jiangsu, China).
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