Total RNA was isolated from GCA tissues, normal tissues as well as MGC80-3, HGC-27 and OE33 cells with Trizol (Invitrogen). Each RNA sample was treated with RNase-Free DNase (Invitrogen) to avoid contamination of genomic DNA. The corresponding cDNAs were generated using the Revert Ace kit (TOYOBO, Osaka, Japan). As described previously 19 (link), human miR-1262 and U6 small RNA were detected with their specific stem-loop RT-PCR primers (Ribobio, Guangzhou, China) using SYBR-Green qRT-PCR. ULK1 and β-actin mRNA expression was examined using their specific qRT-PCR primers (Table S3 ).
Sybr green qrt pcr
Manufactured by RiboBio
Sourced in China
SYBR-Green qRT-PCR is a laboratory equipment used for quantitative real-time reverse transcription polymerase chain reaction (qRT-PCR) analysis. It utilizes the SYBR Green fluorescent dye to detect and quantify target gene expression in RNA samples.
Automatically generated - may contain errors
2 protocols using sybr green qrt pcr
1
Quantifying miRNA and mRNA Levels
2
Reliable RNA Isolation and miRNA Quantification
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Total RNA was isolated from H1299 and PC9 cells with Trizol reagent (Invitrogen). To avoid contamination of genomic DNA, each RNA sample was treated with RNase-Free DNase to remove genomic DNA (Invitrogen). The corresponding cDNAs were generated using the Revert Ace kit (TOYOBO, Osaka, Japan). Human miR-608, miR-4513, and U6 small RNA were examined with their specific stemloop RT-PCR primers (Ribobio, Guangzhou, China) using SYBR-Green qRT-PCR as described previously [28, 29] .
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