Strontium chloride

Manufactured by Merck Group

Sourced in United States, Italy

Strontium chloride is a chemical compound with the formula SrCl2. It is a crystalline solid that is soluble in water and ethanol. Strontium chloride is primarily used in research and laboratory settings as a source of strontium ions.

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Lab products found in correlation

Magnesium chloride, by Merck Group (5 mentions) Calcium chloride, by Merck Group (4 mentions) Sodium hydroxide (naoh), by Merck Group (3 mentions) Boric acid, by Merck Group (3 mentions) Sodium chloride (nacl), by Merck Group (3 mentions) Sodium alginate, by Merck Group (2 mentions) Tris hydroxymethyl aminomethane, by Merck Group (2 mentions) Barium chloride, by Merck Group (2 mentions) Cadmium chloride, by Merck Group (2 mentions) Hydrochloric acid (hcl), by Merck Group (2 mentions) Calcium nitrate tetrahydrate, by Merck Group (2 mentions) Cytochalasin d, by Merck Group (1 mentions) Ethylene glycol tetraacetic acid (egta), by Merck Group (1 mentions) Cytochalasin b, by Merck Group (1 mentions) Four well dishes, by Thermo Fisher Scientific (1 mentions) Calcein am, by Thermo Fisher Scientific (1 mentions) Dexamethasone (dex), by Merck Group (1 mentions) Ascorbic acid, by Merck Group (1 mentions) Triton x 100, by Merck Group (1 mentions) Tri reagent, by Merck Group (1 mentions)

Spelling variants of this product

Strontium chloride hexahydrate (8 citations) Strontium chloride (SrCl2) (5 citations) Strontium chloride hexahydrate SrCl2·6H2O (2 citations)

18 protocols using strontium chloride

Parthenogenetic Activation of Oocytes

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Central and peripheral GV oocytes were in vitro matured for 14 hours (metaphase II). Metaphase II oocytes were parthenogenetically activated by culturing the oocytes for 3 hours with 10 mM strontium chloride (Sigma-Aldrich, no. 255521) and cytochalasin D (5 μg/ml; Sigma-Aldrich, no. C2743) in Ca2⁺/Mg2⁺-free CZB medium. The oocytes were then washed and transferred to kalium simplex optimized medium (KSOM) supplemented with cytochalasin D (5 μg/ml) for an additional 3 hours. The oocytes were then washed and cultured in KSOM for 48 hours before assessing embryo cleavage rates.

Kincade J.N., Hlavacek A., Akera T, & Balboula A.Z. (2023). Initial spindle positioning at the oocyte center protects against incorrect kinetochore-microtubule attachment and aneuploidy in mice. Science Advances, 9(7), eadd7397.

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Diploid Parthenogenetic Oocyte Development

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The oocytes were induced for diploid parthenogenetic development by 6 h incubation in potassium simplex optimized medium (KSOM; MR-121-D, EmbryoMax) supplemented with 5 mM strontium chloride (439665, Sigma-Aldrich), 2 mM EGTA (E3889, Sigma-Aldrich) and

5 μ M

cytochalasin B (C6762, Sigma-Aldrich) in a

{CO}_{2}

-incubator with 5%

{CO}_{2}

and 37°C, as described by Kishigami and Wakayama.²⁰ (link) Then, the oocytes were washed and cultured in KSOM in a

{CO}_{2}

-incubator. The oocytes were observed after 24 and 72 h of incubation to assess the development and calculate two-cell embryos and blastocysts. Laser-treated and control groups were cultured in four-well dishes (Nunc) in separate wells.

Osychenko A.A., Zalessky A.D., Bachurin A.V., Martirosyan D.Y., Egorova M.S, & Nadtochenko V.A. (2024). Stain-free enucleation of mouse and human oocytes with a 1033 nm femtosecond laser. Journal of Biomedical Optics, 29(6), 065002.

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Collagen Type I Extraction and Purification

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Collagen type I was extracted
from the skin of a newborn calf and then purified and dissolved in
an acetic acid solution (pH = 2).^{30 (link)} Sodium
alginate (low viscosity and brown algae), calcium chloride, strontium
chloride, sodium hydroxide (NaOH), ascorbic acid, dexamethasone, DAPI
(4′,6-diamidino-2-phenylindole), PI (propidium iodide), and
tetramethyl rhodamine isocyanate (TRITC)-labeled phalloidin were purchased
from Sigma-Aldrich (USA). Calcein-AM and fluorescent microparticles
were purchased from Invitrogen (USA).

Wei D., Charlton L., Glidle A., Qi N., Dobson P.S., Dalby M.J., Fan H, & Yin H. (2021). Dynamically Modulated Core–Shell Microfibers to Study the Effect of Depth Sensing of Matrix Stiffness on Stem Cell Fate. ACS Applied Materials & Interfaces, 13(32), 37997-38006.

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Osteogenic Differentiation Protocol

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Cytodex 3, Minimum Essential Medium Eagle—Alpha Modification (αMEM), Dulbecco’s modified Eagle medium (DMEM), l-ascorbic acid, β-glycerophosphate, retinoic acid, strontium chloride, oil red O, trypsin-EDTA, phosphate-buffered saline (PBS), glycerol phosphate calcium salt, phosvitin from egg yolk, phosphatase alkaline from bovine intestinal mucose, tris(hydroxymethyl)amino-methane, polyethylene-sorbitan monolaurate (Tween), Triton X-100 and TRI-Reagent were purchased from Sigma Aldrich (St-Louis, USA). Rosiglitazone was obtained from Interchim (Montluçon, France). RiboGreen RNA and PicoGreen DNA quantitation kits were purchased from Invitrogen (Life Technologies, Eugene, OR, USA). RNeasyPlus Mini Kit was purchased from Qiagen (Courtaboeuf, France). Light cycler-FastStart DNA Master, SYBR Green I dye (Lightcycler faststart DNA master SYBR Green I) was obtained from Roche Diagnostics (Meylan, France). Protein assay kit Pierce® BCA (bicinchoninic acid) was obtained from Thermo Scientific (Rockford, USA). Nonidet P-40 substitute was purchased from Fluka Biochemika (Steinheim, Germany). Fetal bovine serum (FBS) was acquired from Biological Industries (Kibbutz Beit Haemek, Israel).

Louis F., Bouleftour W., Rattner A., Linossier M.T., Vico L, & Guignandon A. (2017). RhoGTPase stimulation is associated with strontium chloride treatment to counter simulated microgravity-induced changes in multipotent cell commitment. NPJ Microgravity, 3, 7.

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Synthesis of Biphenyl Derivatives

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We purchased 4,4′-bis(chloromethyl)-1,1′-biphenyl,
(BCMBP, 95%), iron(III) chloride (FeCl₃, 97%), anhydrous
1,2-dichloroethane (DCE, 99%), chlorosulfonic acid (99%), strontium
chloride (SrCl₂, anhydrous powder, >99%), magnesium
chloride (anhydrous >98%), calcium chloride hexahydrate (98%),
and cesium nitrate (>99.99%) from Sigma-Aldrich. Sulfuric acid
(95%) was purchased from Fisher scientific. Sodium chloride (NaCl,
99.9%) was purchased from VWR chemicals and potassium nitrate (KNO₃, > 99%) was purchased from Acros organics. All chemicals
were used as received unless otherwise stated.

James A.M., Harding S., Robshaw T., Bramall N., Ogden M.D, & Dawson R. (2019). Selective Environmental Remediation of Strontium and Cesium Using Sulfonated Hyper-Cross-Linked Polymers (SHCPs). ACS Applied Materials & Interfaces, 11(25), 22464-22473.

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Synthesis and Characterization of Luminescent Ceramic Nanoparticles

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Strontium carbonate (SrCO₃, ≥99.9%), silicon dioxide (SiO₂, 99.5%), boric acid (H₃BO₃, ≥99.5%), europium oxide (Eu₂O₃, 99.99%), dysprosium oxide (Dy₂O₃, 99.99%), magnesium carbonate hydroxide pentahydrate [(MgCO₃)₄·Mg(OH)₂·5H₂O, 99.5%], aluminum oxide (Al₂O₃, 99.99%), zinc sulfide (ZnS, 99.99%), copper(II) acetylacetonate (≥99.9%), manganese(II) carbonate (MnCO₃, ≥99.9%), sulfur (99.98%), calcium carbonate (CaCO₃, ≥99.95%), thulium(III) acetate hydrate (99.9%), sodium citrate dihydrate (≥99%), strontium chloride (SrCl₂, ≥99.99%), magnesium chloride (MgCl₂, 99.9%), and citric acid (≥99.5%) were purchased from Sigma-Aldrich Inc. Ethanol, FBS, hydrochloric acid, nitric acid (HNO₃), 1× PBS, paraformaldehyde, and N,N-dimethylformamide (DMF) were purchased from Thermo Fisher Scientific. Methoxy(polyethylene glycol)-silane (mPEG-silane; 20 kDa) was purchased from Jenkem Technology. All chemicals were used as purchased.

Yang F., Wu X., Cui H., Ou Z., Jiang S., Cai S., Zhou Q., Wong B.G., Huang H, & Hong G. (2022). A biomineral-inspired approach of synthesizing colloidal persistent phosphors as a multicolor, intravital light source. Science Advances, 8(30), eabo6743.

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Lipid Monolayers under Electrolyte Influence

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Triple-distilled water (pH 7; second distillation performed over KMnO₄ and KOH, both from POCh (Polish Chemical Reagents) to remove organic impurities), containing various electrolyte, was used as a subphase for the L-α-phosphatidylcholine from egg yolk monolayer. Electrolyte solutions (concentration range 5.0 × 10⁻⁵–5.0 × 10⁻³ mol dm⁻³) were prepared from triple-distilled water and strontium chloride (SrCl₂, 99%), barium chloride (BaCl₂, 99%), cadmium chloride (CdCl₂, 99.9%), and lead nitrate (Pb(NO₃)₂, 99%), purchased from Sigma-Aldrich (St. Louis, MO, USA). The electrolyte was of p.a. purity and used without further purification. They contained no impurities with surface activity, since the subphases showed zero surface pressure before the spreading of the L-α-phosphatidylcholine from egg yolk.

Petelska A.D, & Naumowicz M. (2017). The effect of divalent ions on L-α-phosphatidylcholine from egg yolk monolayers at the air/water interface. Journal of Biological Inorganic Chemistry, 22(8), 1187-1195.

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Graphene Oxide-Alginate Hydrogel Crosslinking

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Calcium chloride (≥ 93.0%, Sigma-Aldrich), Zinc chloride (≥ 98.0%, Sigma-Aldrich), Strontium chloride(≥ 99.99%, Sigma-Aldrich) as crosslinkers, graphene oxide (Sigma-Aldrich) and sodium alginate (Sigma-Aldrich) were used without further purification.

Serrano-Aroca Á, & Deb S. (2017). Synthesis of irregular graphene oxide tubes using green chemistry and their potential use as reinforcement materials for biomedical applications. PLoS ONE, 12(9), e0185235.

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Biomimetic Titanium Surface Modification

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Commercial pure Ti (99.6%) was purchased from Zhongtian Co., Ltd. (China). Dopamine (dopamine hydrochloride), sodium alginate (Mw~25 kDa), hydrochloric acid, sodium hydroxide, acetone, ethyl alcohol, tris(hydroxymethyl)aminomethane, strontium chloride, 1-ethyl-(3-dimethylaminopropyl)carbodiimide (EDC), and N-Hydroxysuccinimide (NHS) were obtained from Sigma-Aldrich (USA).

Yuan N., Jia L., Geng Z., Wang R., Li Z., Yang X., Cui Z., Zhu S., Liang Y, & Liu Y. (2017). The Incorporation of Strontium in a Sodium Alginate Coating on Titanium Surfaces for Improved Biological Properties. BioMed Research International, 2017, 9867819.

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Synthesis of Sr-Doped Mesoporous Bioactive Glass

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Sr-MBGs nanoparticles have been prepared by using a base-catalysed sol-gel synthesis, following the procedure reported previously by the authors[16 ]. In particular, MBGs with 2% molar percentage of Sr (molar ratio Sr/Ca/Si = 2/13/85, named hereafter as MBG_Sr2%_SG) was prepared as follows: 6.6 g cetyltrimethylammonium bromide (CTAB ≥98%, Sigma Aldrich, Italy) and 12 mL NH4OH (Ammonium hydroxide solution, Sigma Aldrich, Italy) were dissolved in 600 mL of double distilled water (ddH₂O) under stirring for 30 min. Then, 30 mL tetraethyl orthosilicate (TEOS, Tetraethyl orthosilicate, reagent grade 98%, Sigma Aldrich, Italy), 4.888 g of calcium nitrate tetrahydrate (Ca (NO₃)₂·4H₂O, 99%, Sigma Aldrich, Italy) and 0.428 g of strontium chloride (SrCl₂ 99%, Sigma Aldrich, Italy) were added under vigorous stirring for 3 h. The powder was collected by centrifugation (Hermle Labortechnik Z326) at 11,000 rpm for 5 min, washed one time with distilled water and two times with absolute ethanol. The final precipitate was dried at 70 °C for 12 h and calcined at 600 °C in air for 5 h at a heating rate of 1 °C min⁻¹ using a Carbolite 1300 CWF 15/5, in order to remove CTAB. All the reagents were purchased from Sigma Aldrich, Italy and used as received.

Pontremoli C., Izquierdo-Barba I., Montalbano G., Vallet-Regí M., Vitale-Brovarone C, & Fiorilli S. (2019). Strontium-releasing mesoporous bioactive glasses with anti-adhesive zwitterionic surface as advanced biomaterials for bone tissue regeneration. Journal of colloid and interface science, 563, 92-103.

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