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Phospho akt ser473 and thr308

Manufactured by Cell Signaling Technology
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Phospho-Akt (Ser473 and Thr308) is a lab equipment product that detects the phosphorylation of Akt at specific serine and threonine residues. It is used to monitor the activation state of the Akt signaling pathway.

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Lab products found in correlation

Phospho mtor, by Cell Signaling Technology (2 mentions) Phospho h2ax ser139, by Merck Group (1 mentions) Phospho akt, by Cell Signaling Technology (1 mentions) Enhanced chemi luminescence (ecl), by Roche (1 mentions) Hrp conjugated secondary ab, by Cell Signaling Technology (1 mentions) Phospho erk, by Cell Signaling Technology (1 mentions) Gapdh, by Cell Signaling Technology (1 mentions) P70s6k, by Cell Signaling Technology (1 mentions) β actin, by Cell Signaling Technology (1 mentions) β tubulin, by Cell Signaling Technology (1 mentions) Phospho p70s6k, by Cell Signaling Technology (1 mentions)

Close spelling variants of this product

Phospho-Akt (892 citations) Phospho-Akt (Ser473) (647 citations) Phospho-AKT (Thr308) (121 citations) AKT and phospho-AKT(Ser473), (8 citations) (Ser473) Akt (6 citations) Phospho-Akt and Akt (3 citations) Anti-phospho-Akt (Ser473 and Thr308) (2 citations) Rabbit polyclonal anti-phospho Akt Thr308 and Ser473 (2 citations)

2 protocols using phospho akt ser473 and thr308

1

Western Blot Analysis of Signaling Proteins

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The proteins (50 μg of protein/aliquot) in aliquots of cell lysates were separated by sodium dodecylsulfate polyacrylamide gel electrophoresis (SDS-PAGE; 8–15% polyacrylamide), transferred to polyvinylidene difluoride (PVDF) membranes, and immunoblotted with various antibodies. Bound antibodies were detected using appropriate peroxidase-coupled secondary antibodies followed by enhanced chemiluminescence (ECL, Boehringer Mannheim, Mannheim, Germany). Antibodies to phospho-Akt and phospho-mTOR were obtained from Cell Signaling, Inc. (Burlingame, CA, USA), phospho-Akt (Ser473 and Thr308) and phospho-mTOR (Ser2448), phosphor-p70 S6 kinase (Thr389) and caspase-3 from Cell Signaling Technology (Danvers, MA, USA), S6 and beta-actin from Santa Cruz Biotechnology (Santa Cruz, CA, USA), and phospho-H2AX (Ser139) from Millipore Corporation (Billerica, MA, USA). Beta-actin was used as the loading control.
Liu W.L., Gao M., Tzen K.Y., Tsai C.L., Hsu F.M., Cheng A.L, & Cheng J.C. (2014). Targeting Phosphatidylinositide3-Kinase/Akt pathway by BKM120 for radiosensitization in hepatocellular carcinoma. Oncotarget, 5(11), 3662-3672.
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2

Western Blot Analysis of Tbet+ iTreg Cells

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Protein lysates were obtained from Tbet+ iTreg, and Tbet+ iTregPDL1 cells. Lysates were run on 10-20% SDS-PAGE gels and transferred onto nitrocellulose membrane. Membranes were blocked with 5% milk in TBST buffer (20mmol/L TrisHCl, 500 mmol/L NaCl, and 0.01% Tweeen-20) and incubated overnight at 4° C with primary antibodies (Ab) in TBST containing either 5% milk or BSA. Immune reactivity was detected by sequential incubation with HRP-conjugated secondary Ab and enzymatic chemiluminescence (Cell Signaling Technology). Primary Abs to mouse PTEN, mTOR, phospho-mTOR, Akt, phospho-AKT (Ser473 and Thr 308), Foxp3, P70S6K, phospho-P70S6K, ERK, phospho-ERK, GAPDH, β-tubulin, β-actin were procured from Cell Signaling. AEP (Legumain) was obtained from R&D systems. Images were acquired using a LiCOR FcOdyssey system or Wes Simple Protein system.
Stathopoulou C., Gangaplara A., Mallett G., Flomerfelt F.A., Liniany L.P., Knight D., Samsel L.A., Berlinguer-Palmini R., Yim J.J., Felizardo T.C., Eckhaus M.A., Edgington-Mitchell L., Martinez-Fabregas J., Zhu J., Fowler D.H., van Kasteren S.I., Laurence A., Bogyo M., Watts C., Shevach E.M, & Amarnath S. (2018). Programmed death-1 receptor signaling downregulates asparaginyl endopeptidase to maintain Foxp3 stability in induced Regulatory T cells. Immunity, 49(2), 247-263.e7.
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