Quanta lite ccp 3.1 igg iga elisa

Blood samples were taken from the left antecubital vein. Serum lipids [total cholesterol (TC), LDL-cholesterol (LDL-C), HDL-cholesterol (HDL-C), triglycerides (TG)] and glucose levels were measured with a Hitachi 917 analyzer (Roche Diagnostics, Hitachi Ltd., Japan) using standardized laboratory techniques. High-sensitivity C-reactive protein (hsCRP) was measured with immunonephelometry (Nephelometer BM II, Siemens Health Care Diagnostics Inc., USA).
In RA patients, rheumatoid factor (RF) was determined by immunoturbidimetric assay (APTEC Diagnostics nv., ALLmed Diagnostics) and anticyclic citrullinated peptide antibodies (aCCP) with ELISA (QUANTA Lite CCP 3.1 IgG/IgA ELISA, INOVA Diagnostics, Inc., San Diego, USA).

Each serum sample was assessed for rheumatoid factor (RF) and anti-citrullinated peptide antibodies (ACPA). ACPA serology was defined as positive if at least one of the following tests was positive: CCPlus Immunoscan (anti-CCP2) IgG ELISA (Svar Life Science, Malmö, Sweden), QUANTA Lite CCP3.1 IgG/IgA ELISA (INOVA Diagnostics), QUANTA Lite CCP3 IgG ELISA (INOVA Diagnostics) or QUANTA Flash CCP3 IgG CIA (INOVA Diagnostics). Similarly, RF was defined as positive if at least one of the following tests was positive: QUANTA Lite RF IgM ELISA (INOVA Diagnostics), QUANTA Lite RF IgA ELISA (INOVA Diagnostics), Elia RF IgM (Phadia AB) or Elia RF IgA (Phadia AB). Results were recorded in the database for each test and interpreted based on the manufacturer’s recommended cutoffs.