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Transwell insert chambers with an 8 μm pore size

Manufactured by Corning
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Transwell insert chambers with an 8 μm pore size are a type of cell culture insert that allows for the study of cell migration and invasion. The insert chamber has a porous membrane with an 8 μm pore size, which enables the passage of cells through the membrane.

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Lab products found in correlation

Matrigel, by BD (1 mentions)

Close spelling variants of this product

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2 protocols using transwell insert chambers with an 8 μm pore size

1

Transwell Assay for Cell Migration and Invasion

Cited 1 time
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Cell migration and invasion were evaluated using Transwell insert chambers with an 8 μm pore size (Corning, Maine, USA) coated with or without Matrigel (BD Biosciences, Bedford, MA), and 5 × 104 cells were seeded in the serum-free medium in the upper chamber. The lower chamber was filled with 600 μL DMEM with 10% FBS. After 48 h, the cells that had traversed the membrane were fixed, stained in Alexa Fluor 488® phalloidin solution for 30 min, and counted [31 (link)].
Tang Y., Lei Y., Huang S., Li Z., Chen X., Luo H., Cheng C., Chen J., Zou X, & Chen X. (2020). Pristimerin Exacerbates Cellular Injury in Conditionally Reprogrammed Patient-Derived Lung Adenocarcinoma Cells by Aggravating Mitochondrial Impairment and Endoplasmic Reticulum Stress through EphB4/CDC42/N-WASP Signaling. Oxidative Medicine and Cellular Longevity, 2020, 7409853.
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2

Transwell Invasion Assay with Matrigel

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The transwell invasion assay was performed as previously described 13, with modifications. Transwell insert chambers with an 8 μm pore size (Corning) were used to examine the cell invasion ability. Before 1 × 105 cells were seeded in the serum‐free medium in the upper chamber, the transwell filter inserts were coated with Matrigel. After the cells were incubated at 37°C for 24 h, the cells in the upper chamber were removed with a cotton swab. However, the cells that had traversed the membrane were fixed, stained, and counted.
Xu L., Du B., Lu Q., Fan X., Tang K., Yang L, & Liao W. (2018). miR‐541 suppresses proliferation and invasion of squamous cell lung carcinoma cell lines via directly targeting high‐mobility group AT‐hook 2. Cancer Medicine, 7(6), 2581-2591.
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