C57 mdx

Male C57 mdx (n = 12), C57 WT (n = 12), D2 mdx (n = 12), and D2 WT (n = 12) mice were purchased from Jackson Laboratories at 7–8 weeks of age. They were acclimated and housed in Brock University’s Animal Facility, in an environmentally controlled room with a standard 12:12 h light-dark cycle and allowed access to food and water ad libitum. After the mice reached 9–10 weeks of age, they were euthanized via cervical dislocation under general anesthetic (vaporized isoflurane) and their tissues were collected.

Male D2 mdx (8–12 weeks of age, n = 12; stock number = 013141) and male WT D2 mice (n = 12; stock number = 000671) as well as C57mdx (n = 12; stock number = 001801) and C57 WT (n = 12; stock number = 000476) were purchased from Jackson Laboratories. Mice were housed in standard 12 : 12‐h light : dark cycles and were allowed access to standard rodent chow and water ad libitum. All mice were euthanized via exsanguination under general anaesthetic (5% vaporized isoflurane), and the left and right prefrontal cortex and hippocampus were quickly dissected and snap‐frozen in liquid nitrogen and stored in a −80 °C freezer until western blot analyses. All animal protocols were approved by the Brock University Animal Care Committee (AUP 17‐06‐03) and were in compliance with the Canadian Council on Animal Care.