Continuous wavelength multifunctional microplate reader

The biochemical indexes, FINS and 2hPG were detected using the Roche Cobas-8000 automatic biochemical analyzer (Roche Diagnostics, Basel, Switzerland). The serum chemerin level was measured using the ELISA kit (R&D Systems, Minneapolis, MN, USA). After the loading and treatment according to the instructions of kit, the optical density (OD) value at 450 nm was measured using the continuous wavelength multi-functional microplate reader (Tecan, Grödig, Austria), the standard curve was drawn, and the chemerin content in each sample was calculated. The insulin resistance of patients was evaluated via the homeostasis model assessment of insulin resistance (HOMA-IR): HOMA-IR = fasting plasma glucose (FPG) (mmol/l) × FINS (mIU/l)/22.5. Pancreatic β-cell function was evaluated via HOMA-β: HOMA-β = FINS (mIU/l) × 20/[FPG (mmol/l)-3.5].

Dual-energy X-ray absorptiometry (Hologic, Marlborough, MA, USA); fully automatic biochemistry analyzer (Mindray Biomedical Electronics Co., Ltd., Shenzhen, China); continuous-wavelength multifunctional microplate reader (Tecan, Salzburg, Austria).

Instuments used in the study were: Ultramicro-ultraviolet spectrophotometer (Thermo Fisher Scientific, Inc., Waltham, MA, USA); continuous wavelength multi-functional microplate reader (TECAN, Salzburg, Austria); WMT-100 Morris water maze video analysis system (Chengdu Taimeng Science and Technology Co., Ltd., Chengdu, China); and low-temperature high-speed centrifugal machine (Thermo Fisher Scientific, Inc.).