Fc200

Flow cytometry is an effective method to detect cell apoptosis after drug treatment (13 (link)). A specified number of cells of each group were seeded into six-well culture plates. Then cells were treated with 10 µCi/ml of ⁸⁹SrCl₂ for 24 h. Cells were collected and then resuspended in 1× binding buffer at a concentration of 1 × 10⁶ cells/ml. Cell apoptosis analysis was then performed using Annexin-V Apoptosis Detection Kit APC (eBioscience, San Diego, CA, USA) according to the instructions of the manufacturer. Finally, apoptotic cells were analyzed at 488 nm by flow cytometry on a Beckman Coulter FC200 (Fullerton, CA, USA).

A specified number of cells of each group were seeded into six-well culture plates. Then cells were treated with 10 µCi/ml of ⁸⁹SrCl₂ for 24 h. Cells (1 × 10⁶ cells/ml) were harvested by trypsinization, washed by ice-cold PBS, and fixed in ice-cold 70% ethanol. Cells were then incubated with bovine pancreatic RNAase A (500 U/ml; Sigma-Aldrich, St. Louis, MO, USA) at 37°C for 30 min, and then 0.25% propidium iodide (PI; Sigma-Aldrich) was added to stain cells for 30 min at room temperature. Finally, cells in each group were analyzed at 488 nm by flow cytometry on a Beckman Coulter FC200.