Anti phosphoser319 foxo1

Manufactured by Bioss Antibodies

Anti-phosphoSer319 FoxO1 is a monoclonal antibody that recognizes the phosphorylated form of the FoxO1 transcription factor at serine 319. This antibody can be used to detect the phosphorylation status of FoxO1 in various experimental applications.

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Lab products found in correlation

Facsaria 2, by BD (2 mentions) Live dead cell viability assay, by Thermo Fisher Scientific (2 mentions) Lsrfortessa, by BD (2 mentions) Anti lag 3, by R&D Systems (2 mentions) Anti il 10 jes3 9d7, by BioLegend (1 mentions)

2 protocols using anti phosphoser319 foxo1

Comprehensive Immunophenotyping by Flow Cytometry

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For cellular isolation and immunophenotyping analyses, flow cytometry was performed using a BD LSRFortessa for analysis and a BD FACS Aria II for cell sorting. All analysis was carried out using FlowJo software (Treestar Inc). Cell viability was assessed using Live/Dead Cell Viability Assays (Life technologies). The following anti-human surface and intracellular antibodies and dyes were utilized: anti-CD4 (RPA-T4), anti-CD8 (RPA-T8), anti-CD25 (M-A251), anti-CD127 (HIL-7R-M21), anti-PD-1 (EH12.1), anti-PDL1 (MIH1) anti-IFNγ(B27), anti-KI67 (B56), anti-CCR6 (11A9), anti-CXCR3 (IC6), anti-CCR7 (3D12), anti-CXCR5 (RF8B2), V500 Annexin-V stain (all BD Biosciences); anti-TIM-3 (F38-2E2), anti-CTLA-4 (L3D10), anti-CD226 (TX25), anti-IL-10 (JES3-9D7) (all from Biolegend), anti-Foxp3 (PCH101), anti-IL-2 (MQ1-17H12), anti-ICOS (ISA3) (all from eBioscience), anti-LAG-3 (polyclonal goat IgG, R&D systems), anti-phosphoSer319 FoxO1 (polyclonal rabbit IgG, Bioss).

Goods B.A., Hernandez A.L., Lowther D.E., Lucca L.E., Lerner B.A., Gunel M., Raddassi K., Coric V., Hafler D.A, & Love J.C. (2017). Functional differences between PD-1+ and PD-1- CD4+ effector T cells in healthy donors and patients with glioblastoma multiforme. PLoS ONE, 12(9), e0181538.

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Comprehensive Immunophenotyping by Flow Cytometry

Check if the same lab product or an alternative is used in the 5 most similar protocols

For immunophenotyping analyses, flow cytometry was performed with the following anti-human surface and intracellular antibodies and dyes: anti-CD4 (RPA-T4), anti-CD8 (RPA-T8), anti-CD25 (M-A251), anti-CD127 (HIL-7R-M21), anti-PD-1 (EH12.1), anti-PDL1 (MIH1) anti-IFN-γ (B27), anti-KI67 (B56), anti-CCR6 (11A9), anti-CXCR3 (IC6), anti-CCR7 (3D12), anti-CXCR5 (RF8B2), and V500 Annexin-V stain (all from BD Biosciences); anti-TIM-3 (F38-2E2), anti-CTLA-4 (L3D10), anti-CD226 (TX25), and anti-IL-10 (JES3-9D7) (all from Biolegend); anti-Foxp3 (PCH101), anti-IL-2 (MQ1-17H12), and anti-ICOS (ISA3) (all from eBioscience); anti-LAG-3 (polyclonal goat IgG, R&D Systems); and anti-phosphoSer319 FoxO-1 (polyclonal rabbit IgG, Bioss). Cell viability was assessed using Live/Dead Cell Viability Assays (Life technologies). Samples were run using a BD LSRFortessa for all analysis, and FACS sorting was carried out on a BD FACS Aria II. FlowJo software (TreeStar Inc.) was used for data collection and analysis.

Lowther D.E., Goods B.A., Lucca L.E., Lerner B.A., Raddassi K., van Dijk D., Hernandez A.L., Duan X., Gunel M., Coric V., Krishnaswamy S., Love J.C, & Hafler D.A. (2016). PD-1 marks dysfunctional regulatory T cells in malignant gliomas. JCI Insight, 1(5), e85935.

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