Anti nup214

Manufactured by Abcam

Sourced in China

Anti-Nup214 is a primary antibody that recognizes the Nup214 protein, also known as CAN. Nup214 is a component of the nuclear pore complex which facilitates the transport of molecules between the nucleus and cytoplasm. This antibody can be used to detect and study Nup214 expression and localization.

Automatically generated - may contain errors

Lab products found in correlation

Anti mouse igg hrp, by Thermo Fisher Scientific (1 mentions) Ecl detection kit, by GE Healthcare (1 mentions) Mouse monoclonal anti ha, by Santa Cruz Biotechnology (1 mentions) Monoclonal mouse anti actin, by MyBioSource (1 mentions) Anti rabbit igg hrp, by Thermo Fisher Scientific (1 mentions) Anti laci, by Merck Group (1 mentions) Anti nup98, by Cell Signaling Technology (1 mentions)

2 protocols using anti nup214

Quantification of Viral and Cellular Proteins

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The expression levels of Nup214, Nup214ct, and viral proteins in transfected and/or virus-infected cells were analyzed with Western blotting. The cells grown in 12- or 24-well plates were lysed in an SDS-PAGE sample buffer. The proteins in lysates were separated using SDS-PAGE and transferred to a polyvinylidene difluoride (PVDF) membrane. After blocking, the membrane was exposed to the specific primary antibodies [monoclonal mouse anti-HA (Santa Cruz Biotechnology, Dallas, TX, USA; #sc-7392), monoclonal mouse antiactin (MyBioSource, San Diego, CA, USA; #u1d3e), rabbit polyclonal anti-Nup214 (Abcam, Shanghai, China; #ab70497), polyclonal rabbit anti-NS2 (Invitrogen, #PA5-32234), and anti-M1 polyclonal rabbit antisera] overnight at 4 °C and then to a horseradish peroxidase-conjugated second antibody [antimouse IgG-HRP (Invitrogen, #31420) and/or antirabbit IgG-HRP (Invitrogen, #31423)] against species-specific immunoglobulins for 45 min at room temperature. The proteins were visualized with an ECL detection kit (GE Healthcare, Milan, Italy).

ŞENBAŞ AKYAZI B., PİRİNÇAL A., KAWAGUCHI A., NAGATA K, & TURAN K. (2020). Interaction of influenza A virus NS2/NEP protein with the amino-terminal part of Nup214. Turkish Journal of Biology, 44(2), 82-92.

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Antibodies for Nucleoporin Analysis

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Antibodies used in this study included rabbit anti-Nup133–555 conjugated,⁴⁰ (link) anti-Nup160,⁴⁰ (link) anti-Nup53, anti-Nup85,⁴⁰ (link) anti-Nup62 (Sigma-Aldrich, SAB1410438), anti-Nup214 (Abcam, ab84357), Anti-Nup98 (Cell Signaling Technology, #2598), anti-Nup93,⁸³ (link) anti-Pom121 (Gene-Tex, GTX102128), anti-ELYS (Bethyl Laboratories, A300–166A), and mouse anti-FG Nup mAb414 (Biolegend, #mms-120p) and anti-LacI (Merck Millipore, 05–503).

Schwartz M., Travesa A., Martell S.W, & Forbes D.J. (2015). Analysis of the initiation of nuclear pore assembly by ectopically targeting nucleoporins to chromatin. Nucleus, 6(1), 40-54.

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