Abs used were: p62/SQSTM1 (Cell Signaling Technology, Danvers, MA; #5114; 1:1000
in 5% BSA), Pellino1 (Cell Signaling Technology; #31474; 1:1000)in 5% BSA), Nrf2
(Thermo Fisher Scientific, Waltham, MA; #PA52788, 1:500 in 5% BSA), and GAPDH
(Cell Signaling Technology; #2118; 1:1000 in 5% BSA). All blots were detected
using an anti-rabbit IgG-HRP-linked Ab (Cell Signaling Technology; #7074; 1:2000
in 5% BSA). Primers were from Integrated DNA Technologies (Coralville, IA) and
were against M. musculus MCP-1, forward: 5′-GCT GTA GTT TTT GTC
ACC AAG CTC-3′, reverse: 5′-AGT GCT TGA GGT GGT TGT GG-3′, and Mus
musculus Slc40a1 (FPN), forward 5′-GAT GGG TCC TTA CTG TCTGCT-3′,
reverse: 5′-GAT TGT GAT GCG AGT GGC AG-3′. LPS was from Escherichia
coli O111:B4 purified by phenol extraction from Sigma–Aldrich (St.
Louis, MO; #L2630) and reconstituted in PBS.
in 5% BSA), Pellino1 (Cell Signaling Technology; #31474; 1:1000)in 5% BSA), Nrf2
(Thermo Fisher Scientific, Waltham, MA; #PA52788, 1:500 in 5% BSA), and GAPDH
(Cell Signaling Technology; #2118; 1:1000 in 5% BSA). All blots were detected
using an anti-rabbit IgG-HRP-linked Ab (Cell Signaling Technology; #7074; 1:2000
in 5% BSA). Primers were from Integrated DNA Technologies (Coralville, IA) and
were against M. musculus MCP-1, forward: 5′-GCT GTA GTT TTT GTC
ACC AAG CTC-3′, reverse: 5′-AGT GCT TGA GGT GGT TGT GG-3′, and Mus
musculus Slc40a1 (FPN), forward 5′-GAT GGG TCC TTA CTG TCTGCT-3′,
reverse: 5′-GAT TGT GAT GCG AGT GGC AG-3′. LPS was from Escherichia
coli O111:B4 purified by phenol extraction from Sigma–Aldrich (St.
Louis, MO; #L2630) and reconstituted in PBS.