Adult Wistar rats were obtained from Charles River (Sulzfeld, Germany), bred and maintained at the local animal center in breeding cages under controlled environmental conditions (12 h light/dark cycle, 20–23 °C, 50% relative humidity, drinking and feeding ad libitum). Newborn or up to two-day-old male and female offspring were used for the in vitro model. All animal experiments have been approved by the local animal care and use committee (TS-1/13) and were in accordance with the European Communities Council Directive and German Animal Welfare Act (54–2532.1-23/09, Directive 2010/63/EU).
Adult wistar rats
Manufactured by Charles River Laboratories
Sourced in Germany
Adult Wistar rats are a commonly used laboratory animal model. They are a strain of albino rats that are widely used in medical research and scientific experimentation. The core function of adult Wistar rats is to serve as a model organism for various types of research and testing.
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Lab products found in correlation
Basic fibroblast growth factor (bfgf), by Thermo Fisher Scientific (1 mentions)
Collagenase 2, by Harvard Bioscience (1 mentions)
Its x, by Thermo Fisher Scientific (1 mentions)
Epidermal growth factor (egf), by Thermo Fisher Scientific (1 mentions)
Adult balb c mice, by Charles River Laboratories (1 mentions)
C57bl 6 adult mice, by Charles River Laboratories (1 mentions)
9 protocols using adult wistar rats
1
In Vitro Model Using Newborn Wistar Rats
2
Adult Wistar Rat Maintenance
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Adult Wistar rats (n = 48; 3 months, female, Charles River Laboratories, Barcelona, Spain) were maintained on a 12 h light/dark cycle with food and water ad libitum at the Universidad of Castilla-La Mancha Animal house (Albacete, Spain). The procedures involving the use and care of the animals were approved by the corresponding Institutional Animal Care and Use Committee (Permit Number: PR-2013-02-03). These protocols were in accordance with the guidelines of the European Communities Council (Directive 2010/63/EU) and current national legislation (R.D. 53/2013; Law 32/2007).
3
Maternal Immune Challenge in Rats
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Adult Wistar rats (females 226–250 g and males 251–275 g upon arrival) were purchased from Charles River (Sulzfeld, Germany). The animals were maintained under standard conditions: room temperature of 23 °C, 12/12-h light/dark cycle, lights on at 6:00 am, and ad libitum access to water and food. The phase of the oestrous cycle was determined based on vaginal smears that were obtained daily from the females. On the day of pro-oestrus, the females were placed with males for 12 h, and the presence of sperm in vaginal smears was checked the next morning [defined as gestational day 1 (GD1)]. Pregnant females (n = 28) were randomly divided into four equal groups: (1) control for LPS (kLPS), (2) control for Poly I:C (kPoly), (3) LPS and (4) Poly I:C. All procedures were approved by the Animal Care Committee of Maj Institute of Pharmacology, Polish Academy of Sciences, Cracow, and met the criteria of the International Council for Laboratory Animals and Guide for the Care and Use of Laboratory Animals (consent number: 236/2016). All possible efforts were made to minimize the number of animals used and their suffering.
4
Maternal Immune Activation in Rats
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Adult Wistar rats were purchased from Charles River (Sulzfeld, Germany) and housed under standard conditions with a room temperature of 23 °C, 12/12 h light/dark cycle (lights on at 6:00 am) and ad libitum access to water and food. A set of pregnant females (n = 22) was generated as previously described [30 (link),33 (link)] and randomly divided into two equal groups: (1) control and (2) MIA. All procedures were performed under the approval of the Animal Care Committee of the Maj Institute of Pharmacology, Polish Academy of Sciences, Cracow and followed the recommendations of the International Council for Laboratory Animal Science and Guide for the Care and Use of Laboratory Animals (consent numbers: 236/2016 and 128/2018). All possible efforts were made to minimize the number of animals used and their suffering.
5
Isolation and Expansion of Skeletal Myocytes
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Adult Wistar rats (Charles River) were euthanized in CO2 anesthesia by cervical dislocation. Organ harvest was approved by the Niedersächsisches Landesamt für Verbraucherschutz und Lebensmittelsicherheit (LAVES: AZ 10/13). For one series of ESM skeletal muscle from both hind limbs (total 20‐30 g) of one Wistar rat (6‐8 weeks old) was collected in basal medium (DMEM/F12, 5% fetal bovine serum [FBS], 2 mmol/L glutamine, 100 U/ml penicillin, 100 µg/ml streptomycin) on ice. Connective tissue and tendons were removed and the muscle was minced to a fine slurry. This was digested for 90 min at 37°C using 2.5 mg/ml Collagenase II (Biochrom) and 20 µg/ml DNAse I (Calbiochem) in basal medium. The tissue suspension was subsequently passed through filters with gradually decreasing mesh size (250 100 40 µm, BD Biosciences). The cell suspension was then preplated for 1 hour in basal medium on cell culture dishes to reduce the number of fibroblasts. The non‐attached cells were resuspended in proliferation medium (basal medium supplemented with 10 ng/ml bFGF; PeproTech), 10 ng/ml EGF (PeproTech), Insulin‐Transferrin‐Selenite (ITS) + X (100x; Invitrogen) and expanded for 5 days on uncoated flasks without further passaging. For implantation experiments transgenic Lewis rats with ubiquitous expression of EGFP were used for skeletal myocyte preparation.28
6
Wistar Rat Breeding and Care
7
Rat SCI Neuroprotection Treatments
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Adult Wistar rats, male, weighing 275-300g, were purchased from Charles River (Wilmington, MA). All animals were maintained according to National Institutes of Health (NIH) guidelines, and all experimental protocols were approved by the Institutional Animal Care and Use Committee (IACUC) of the University of Maryland School of Medicine. Every effort was made to reduce the total number of rats and their discomfort and pain. 80 rats were randomly divided into five groups: 20 rats in the control group, 20 rats in the SCI group, 5 rats in the SCI+CQ (chloroquine) group, 20 rats in the SCI+TRE (trehalose) group, and 15 rats in the SCI+TRE+CQ group. For two weeks before surgical procedures, all rats were housed in a room with a 12 : 12 h light/dark cycle and free access to regular food and water.
8
Animal Husbandry and Experimental Procedures
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Adult BALB/c mice (20 g) and adult Wistar rats (200 g) were obtained from the Charles River Laboratories (Beijing, China). All procedures were performed in accordance with the guiding principles in the Care and Use of Animals and approved by the Capital Medical University Committee on the Use of Animals in Research and Education. Animals were separately housed in plastic cages in a room maintained at 23.6°C and 35% humidity with 12-hour light/dark cycles (light on at 07:00 AM). Each animal was used only once and fed a standard chow diet with unrestricted water intake. Experiments were conducted in an ABSL-2 laboratory and at the end of the experiments, the animals were anesthetized using pentobarbital sodium and then euthanized.
9
Calretinin Knockout Mice and Rat Study
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Adult Wistar rats (3-6 months old) and adult C57Bl/6 mice (3-5 months old) were purchased from Charles River Laboratories (France). Calretinin knockout (CR -/-) mice were generated on a mixed 129/Ola x C57Bl/6 genetic background, as described previously [19] . CR -/- mice were backcrossed into the C57Bl/6 genetic background with a minimum of 10 generations so that C57Bl/6 mice can be considered as control mice (wild-type, WT). All experiments were carried out comparing WT with CR -/-mice. Mice and rats were housed in a climatecontrolled room with 12 h light -12 h dark periods. Animals were fed with a standard rodent diet and received water ad libitum. The animals were euthanized by cervical dislocation (mice) or concussion followed by decapitation (rats). The laboratory animal care and experimental protocols (CEBEA n°: 494 N, 495 N) were approved by the "Ethical and Animal Welfare Committee" of the Université libre de Bruxelles.
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