MG132 (HY-13259), Z-VAD-FMK (HY-16658B), erastin (HY-15763), 3-MA (HY-19312), Fer-1 (HY-100579), necrostatin-1 (HY-15760), cycloheximide (HY-12320) and RSL3 (HY-100218A) were obtained from MedChemExpress. JNJ-64619178 (S8624) and GSK3326595 (S8664) were purchased from Selleck Chemicals. Mouse anti-PD-1 antibody (BE0146) and IgG control (BE0089) were purchased from Bio X Cell. Recombinant murine IFN-γ (P6137) was purchased from Beyotime. PRMT5 (D5P2T), actin (8H10D10), and symmetric di-Methyl arginine motif (13222) were purchased from Cell Signaling Technology (Massachusetts, USA). KEAP1 antibody (10 503–2-AP), NRF2 antibody (16 396–1-AP), HMOX1 antibody (10 701–1-AP), V5 antibody (14 440–1-AP), HA antibody (51 064–2-AP), TRIM25 antibody (12 573–1-AP), FLAG antibody (20 543–1-AP), SLC7A11 antibody (26 864–1-AP), and GPX4 antibody (67 763–1-Ig) were purchased from Proteintech.
Nrf2 antibody
Manufactured by Proteintech
Sourced in China, United States
Nrf2 antibody is a laboratory tool used to detect the transcription factor Nrf2 in biological samples. Nrf2 is a key regulator of the cellular antioxidant response. The antibody can be used in various applications such as Western blotting, immunohistochemistry, and immunoprecipitation to study the expression and localization of Nrf2 in different cell types and tissues.
Automatically generated - may contain errors
Lab products found in correlation
Keap1 antibody, by Proteintech (4 mentions)
Ho 1 antibody, by Proteintech (4 mentions)
Gapdh antibody, by Proteintech (3 mentions)
Nqo 1 antibody, by Abcam (2 mentions)
Ne per nuclear and cytoplasmic extraction reagent, by Thermo Fisher Scientific (2 mentions)
Rsl3 hy 100218a, by MedChemExpress (1 mentions)
Hmox1 antibody, by Proteintech (1 mentions)
Gsk3326595 s8664, by Selleck Chemicals (1 mentions)
Trim25 antibody, by Proteintech (1 mentions)
Gpx4 antibody, by Proteintech (1 mentions)
Goat serum, by ZSGB-BIO (1 mentions)
Fluorescent secondary antibody, by Proteintech (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Nrf2 phospho s40, by Abcam (1 mentions)
Nqo1 antibody, by Proteintech (1 mentions)
Rpmi 1640, by Thermo Fisher Scientific (1 mentions)
Tlr4 antibody, by Proteintech (1 mentions)
Ab 8 macroporous resin, by Yuanye Bio-Technology (1 mentions)
Streptozocin stz, by Merck Group (1 mentions)
Acetonitrile, by Merck Group (1 mentions)
9 protocols using nrf2 antibody
1
Modulation of Cell Death Pathways
2
Nrf2 Immunofluorescence Staining in Macrophages
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Primary peritoneal macrophages were fixed in 4% paraformaldehyde for 15 h, permeabilized with 0.5% (v/v) Triton X-100 for 20 min, blocked with goat serum (ZSGB Bio, China) for 30 min, and then incubated with Nrf2 antibody (1:100, Proteintech, China) overnight at 4 ℃. After washing with PBST, samples were incubated with fluorescent secondary antibody (1:100, Proteintech, China) in PBST for 1 h at room temperature, and DAPI was used to stain cell nuclei for 5 min and observed under the fluorescence microscope (Nikon Ti-s, Tokyo, Japan).
3
Oxidative Stress Signaling Pathway
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RPMI 1640 and fetal bovine serum were purchased from Gibco-BRL (Gibco-BRL, Carlsbad, California, USA). Active oxygen detection kit (S0033), total SOD active detection kit (S0101), and lipid oxidation detection kit (S0131) were purchased from Full-Style Gold (Beijing, China). The enhanced BCA protein kit (P0009) and nuclear cytoplasmic protein extraction kit (P0028) were purchased from Beyotime Biotechnology (Shanghai, China). Keap1 antibody (catalog number 60027-1-lg), Nrf2 antibody (catalog number 66504-1-lg), NQO1 antibody (catalog number 67240-1-lg), and HO-1 antibody (catalog number 67643-1-lg) are available for purchase from Proteintech (USA). Nrf2 (phospho S40) (catalog number EP1809Y) was purchased from Abcam (UK). β-Actin (Cat. No. 12620), anti-rabbit IgG HRP conjugate (V7951), and anti-mouse IgG HRP conjugate (W4021) were purchased from Promega Corporation (Madison, W1, USA). The inhibitor ML385 (catalog number HY-100523) was purchased from MCE (Shanghai, China). All other reagents for this study were of analytical grade.
4
Hepatoprotective Effects of HTF
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The HTF was obtained by the method of Cheng et al. with some modifications. 95% ethanol (analytical-grade) was purchased from the Chengdu Chron Chemicals Co., Ltd. (Chengdu, Sichuan, China). The AB-8 macroporous resin was purchased from Yuanye Bio-Technology Co., Ltd. (Shanghai, China). Cytokines in serum and liver (interleukin-1β (IL-1β), IL-6 and tumor necrosis factor-α (TNF-α)) were measured using commercial kits purchased from Nanjing Jiancheng Bioengineering Institute (Nanjing, China). Both serum and liver biochemical indicators (high-density lipoprotein (HDL-C), low-density lipoprotein (LDL-C), triglyceride (TG), total cholesterol (TC), glutamic oxalacetic transaminase (GOT), glutamic pyruvic transaminase (GPT)) were measured using Nanjing Jiancheng commercial kits. Antibodies in the experiment were purchased from Proteintech Group, Inc. (Wuhan, Hubei, China), including the Nrf2 antibody, HO-1 antibody, TLR4 antibody, MyD88 antibody, GAPDH antibody, and Keap1 antibody.
5
Diabetic Nephropathy Therapeutic Protocol
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Losartan potassium tablets were purchased (Merck Sharp & Dohme (Australia) Pty., Ltd, South Granville, Australia, import drug registration nos. H20160398 and H20160401); streptozocin (STZ) was purchased from Sigma Chemical Co., St. Louis, USA, CAS no. 18883-66-4; Keap1 antibody was bought from Proteintech Group, Wuhan, China, no. 13161-1-ap; Nrf2 antibody was purchased from Proteintech Group, Wuhan, China, no. 16396-1-ap; heme oxidase-1 (HO-1) antibody was purchased from Proteintech Group, Wuhan, China, no. 10701-1-ap; the malondialdehyde (MDA) test box was purchased (Nanjing Jiancheng Bioengineering Institute, Nanjing, China, no. A003-1). Acetonitrile was purchased from Merck, Billerica, USA; asiaticoside reference was purchased from the National Institute for the Control of Pharmaceutical and Biological Products, Beijing, China; astragaloside reference was purchased from the National Institute for the Control of Pharmaceutical and Biological Products, Beijing, China; triptolide reference was purchased (content ≥98%, the National Institute for the Control of Pharmaceutical and Biological Products, Beijing, China).
6
Ferroptosis-related Protein Regulation
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Ferric ammonium citrate (USA, 1185-57-5) was purchased from Sigma–Aldrich. Recombinant mouse IL-1β (Minneapolis, USA, # 401-ML) was purchased from R&D systems. Erastin (USA, S7242) and Ferrostatin-1 (USA, S7243) were purchased from Selleck. Reactive Oxygen Species Assay Kit (China, S0033) was purchased from Beyotime. C11 BODIPY Lipid Peroxidation Sensor (USA, D3861) was purchased from ThermoFisher. GPX4 (UK, ab125066, diluted 1:5000) antibody, P53 antibody (UK, ab131442, diluted 1:1000), SLC7A11 antibody (UK, ab175186, diluted 1:5000), ACSL4(UK, ab155282, diluted 1:10,000) antibody, Collagen II (UK, ab34712, diluted 1:200 for immunohistochemical staining) antibody, Collagen II (UK, ab185430, diluted 1:1000 for western blot) antibody, NQO-1 antibody (UK, ab80588, diluted 1:10,000), and Trx (UK, ab133524, diluted 1:10,000) antibody were purchased from Abcam. Nrf2 antibody (USA, 16396-1-AP, diluted 1:1000), HO-1 antibody (USA, 10701-1-AP, diluted 1:1000), Beta-actin antibody (USA, 66009-1-Ig, diluted 1:1000) and GAPDH antibody (USA, 60004-1-Ig, diluted 1:1000) were purchased from Proteintech.
7
Investigating Inflammatory Pathways
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The reagents used are as follows: Nrf2 antibody, Proteintech, lot number: 16396-1-AP; NLRP3 antibody, Abcam, lot number: ab263899; Caspase-1 antibody, Servicebio, lot number: GB11383; GSDMD antibody, Abcam, lot number: ab219800; β-actin antibody, Proteintech, lot number: 20536-1-AP; and TUNEL kit: Proteintech, lot number: PF00006.
8
Therapeutic Effects of MPS and VO-OHpic
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MPS was purchased from Pfizer and dissolved in sterile normal saline. VO-OHpic was purchased from selleck (USA, S8174) and dissolved in DMSO. And the final concentration of DMSO is the same (0.1%) in each group including the control. CD31 antibody (UK, ab182981), CD34 antibody (UK, ab81289), VEGF Receptor 2 (VEGFR2) antibody (UK, ab11939), VEGF Receptor 1 (VEGFR1) antibody (UK, ab32152), CD133 antibody (UK, ab19898), SIRT-1 antibody (UK, ab189494), NQO-1 antibody (UK, ab80588), and Trx antibody (UK, ab133524) were purchased from Abcam. FITC-conjugated mouse anti-rabbit IgG antibody was purchased from Boster (China, BM2012). Bad antibody (USA, #9268, for IP), Phospho-Bad antibody (USA, #5284), Bcl-xL antibody (USA, #2764), AKT antibody (USA, #4691), p-AKT antibody (USA, #4060), cleaved caspase-3 (Asp175) antibody (USA, #9661), cleaved caspase-9 antibody (USA, #9508), MFF antibody (USA, #84580), and normal rabbit IgG (USA, #2729) were purchased from Cell Signaling Technology. Bax antibody (USA, 50599-2-Ig, for western blot), Cytochrome C antibody (USA, 66264-1-Ig), Nrf2 antibody (USA, 16396-1-AP), Bcl-xL antibody (USA, 26967-1-AP), Bad antibody (USA, 10435-1-AP), FIS1 antibody (USA, 10956-1-AP), VEGF antibody (USA, 19003-1-AP), HO-1 antibody (USA, 10701-1-AP), and GAPDH antibody (USA, 60004-1-Ig) were purchased from Proteintech.
9
Nrf2 Immunoprecipitation and Western Blot
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MSCs were lysed using NE-PER™ Nuclear and Cytoplasmic Extraction Reagents (Thermo Scientific, 78833), lysates (1 mg protein) were pre-cleared with 20 µL of Protein G Agarose beads (Santa Cruz) for 2–4 h, and then the supernatants were collected by centrifugation at 3,000 rpm at 4 °C for 3 min. The supernatants mixed with the Nrf2 antibody and beads were rotated overnight at 4 °C. After being washed with NP-40 buffer for three times, the immunocomplexes were eluted by boiling in 1× SDS-loading buffer for 10 min. Then, the buffer was separated using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and electroblotted onto a polyvinylidene fluoride (PVDF) membrane (Roche, Basel, Switzerland) in line with standard procedures. Transferred blots were incubated sequentially with the STC2 antibody (Aabcam, UK) and Nrf2 antibody (Proteintech, USA) at 4 °C for 12 hours, and HRP-conjugated secondary antibodies. Protein bands were visualized with an enhanced chemiluminescence detection kit and recorded on a radiographic film (Alpha Innotech, San Jose, CA, USA).
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