Q exactive hybrid quadrupole mass spectrometer

Manufactured by Thermo Fisher Scientific

Sourced in United States

The Q Exactive Hybrid Quadrupole Mass Spectrometer is a high-resolution mass spectrometer that combines a quadrupole and an Orbitrap mass analyzer. It is designed to provide accurate mass measurements and high-resolution data for a wide range of applications in analytical chemistry and life sciences.

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Lab products found in correlation

Ascend 400 spectrometer, by Bruker (1 mentions) Xcalibur 2.2 software suite, by Thermo Fisher Scientific (1 mentions) Fei nova nano 450, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

Thermo Scientific Q Exactive Hybrid Quadrupole Mass Spectrometer ESI-Q Exactive Hybrid Quadrupole-Orbitrap mass spectrometer Q‐Exactive™ H‐X Hybrid Quadrupole‐Orbitrap™ mass spectrometer Q-Exactive hybrid quadrupole orbitrap high resolution mass spectrometer Q-exactive high-field hybrid quadrupole orbitrap mass spectrometer LC–ESI–MS-MS Q Exactive HF hybrid quadrupole-Orbitrap mass spectrometer Q Exactive HF-X Hybrid Quadrupole-Orbitrap Mass Spectrometer Thermo Q Exactive Hybrid Quadrupole-Orbitrap Mass Spectrometer Q Exactive UHMR Hybrid Quadrupole-Orbitrap Mass Spectrometer Thermo Q Exactive HF Hybrid Quadrupole-Orbitrap Mass Spectrometer

3 protocols using q exactive hybrid quadrupole mass spectrometer

Chalcone Derivatives Synthesis and Evaluation

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The molecular docking was performed by using DS-CDocker implemented in Discovery Studio (version 4.5). Melting points of the synthesized compounds (2a–2v) were measured using XT-4 Binocular Microscope (Beijing Tech. Instrument, China) without correction. ¹H-NMR, ¹³C-NMR and ¹⁹F-NMR spectra were obtained on a Bruker Ascend-400 spectrometer (Bruker Optics, Switzerland) and DMSO-d₆ or CDCl₃ were used as a solvent and TMS was used as an internal standard. HRMS data were obtained using Thermo Scientific Q Exactive Hybrid Quadrupole Mass Spectrometer (Thermo Scientific Inc., St Louis, MO, USA). The microscale thermophoresis (MST) of the compounds to check the interaction with TMV-CP (Tobacco mosaic virus coat protein) was determined using a micro thermophoresis instrument (NanoTemper Technologies GmbH, Germany). All reagents and solvents were purchased from Chinese Chemical Reagent Company and were chemically pure analytical grade reagents. The synthetic route of chalcone derivatives containing thiophene sulfonate is shown in Scheme 1. The intermediate 1 were prepared according to the methods already reported in the literature.^{44 (link)}

Guo T., Xia R., Chen M., He J., Su S., Liu L., Li X, & Xue W. (2019). Biological activity evaluation and action mechanism of chalcone derivatives containing thiophene sulfonate. RSC Advances, 9(43), 24942-24950.

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Native MS Analysis of Nt17-Lipid Vesicle Complexes

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Native MS [44 (link)] was used to investigate complexes formed between Nt17 peptide and various lipid vesicles [45 (link)]. Lipid vesicles were formed as previously described, but films were reconstituted in HPLC-grade water instead of tris buffer. Nt17 peptide (20 μM) was incubated with lipid vesicles (20:1 lipid:peptide) for 5 h at 37 °C. Samples were analyzed using a Q Exactive Hybrid Quadrupole mass spectrometer (Thermo Fischer, San Jose, CA) equipped with a commercial HESI source. Spectra were recorded in positive-ion mode over a mass-to-charge (m/z) ratio range of 400 to 4,000, and samples that included anionic lipids were also recorded in negative-ion mode. Samples were infused at a rate of 10 uL/min and the needle was biased at 3,500 V relative to the mass spectrometer inlet. The parameters for the MS instrument were: 400 °C for the capillary inlet temperature, 30 °C for the analyzer temperature, 80 V for the S-lens assembly, 400 ms for the maximum injection time, 1 × 10⁶ for the AGC, and 70,000 for the MS resolution. Each spectrum was recorded in triplicate for 90 s each, and the data was analyzed using the Xcalibur 2.2 software suite (Thermo Scientific).

Beasley M., Groover S., Valentine S, & Legleiter J. (2020). Lipid headgroups alter huntingtin aggregation on membranes. Biochimica et biophysica acta. Biomembranes, 1863(1), 183497.

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Evaluation of Novel Compounds' Properties

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The melting point was measured on an XT-4 binocular microscope (Beijing Taike Instruments Co., Ltd., Beijing, China), without calibration. ¹H, ¹³C and ¹⁹F were obtained by using a 500 MHz nuclear magnetic resonance (NMR) instrument (JEOL-ECX500, Japan Electronics Co., Tokyo, Japan). High-resolution mass spectra (HRMS) were obtained by using a Thermo Scientific Q Exactive Hybrid Quadrupole Mass Spectrometer (Thermo Scientific, St. Louis, MO, USA). Scanning electron microscopy (SEM) data were obtained on FEI Nova Nano 450 (FEI Co., Hillsboro, OR, USA). The cell permeability was measured on the conductivity meter, Leici DDSJ-3O8F (Shanghai Instrument & Electric Science Instrument Co., Ltd., Shanghai, China), and the cell leakage was recorded on the N-5000 ultraviolet spectrophotometer (Shanghai Yoke Instrument Co., Ltd., Shanghai, China). The reagents and solvents used in the experiment were purchased from Shanghai Titan Chemical Co., Ltd. (Shanghai, China), Beijing Solarbio Technology Co., Ltd. (Beijing, China) and Tianjin Zhiyuan Chemical Reagent Co., Ltd. (Tianjin, China). All reagents and solvents used were analytical grade, and they were directly used without further purification or drying.

Zhou R., Zhan W., Yuan C., Zhang T., Mao P., Sun Z., An Y, & Xue W. (2023). Design, Synthesis and Antifungal Activity of Novel 1,4-Pentadiene-3-one Containing Quinazolinone. International Journal of Molecular Sciences, 24(3), 2599.

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