2 4 dinitrophenylhydrazine 2 4 dnp

Manufactured by Merck Group

2,4-dinitrophenylhydrazine (2,4-DNP) is a chemical reagent commonly used in analytical and organic chemistry applications. It is a yellow crystalline solid with a melting point of 198-202°C. 2,4-DNP is primarily used as a derivatizing agent to detect and identify carbonyl compounds through the formation of stable hydrazones.

Automatically generated - may contain errors

Lab products found in correlation

Phenyl methyl sulfonyl fluoride (pmsf), by Merck Group (1 mentions) Amylase resin, by New England Biolabs (1 mentions) Ni nta, by New England Biolabs (1 mentions) Pmal c2x, by New England Biolabs (1 mentions) Ptwin1, by New England Biolabs (1 mentions) Ni nta superflow, by Qiagen (1 mentions) Dl dap, by Merck Group (1 mentions) Alcohol dehydrogenase from saccharomyces cerevisiae, by Merck Group (1 mentions)

Close spelling variants of this product

2,4-dinitrophenylhydrazine (119 citations) Dinitrophenylhydrazine (18 citations) 2,4-DNP (15 citations)

2 protocols using 2 4 dinitrophenylhydrazine 2 4 dnp

Recombinant Amylase Purification Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

Amylase resin, the plasmids pMAL-c2x and pTWIN1 are products of NEB, USA. Ni-NTA and proteases including factor Xa, enterokinase and thrombin are bought from Novagen, USA. E. coli strain BL21(DE3), and pET28b and pCDF-Duet1 vectors, and reagents for Western blot analysis are supplied by Novagen, USA. Ni-NTA superflow was obtained from Qiagen (Chatsworth, CA). Ultra-15 centrifugal filter tube equipped with Ultracel-10 membrane was supplied by Amicon (USA). The compounds including 2,4-dinitrophenylhydrazine (2,4-DNP), PLP and DL-DAP, PMSF, NEM, BzCl were from Sigma. Primer synthesis and DNA sequencing were performed by Invitrogen (Shanghai, China). Reagents used in plasmid construction and protein expression were from Takara (Dalian, China). The purified GST fused R3P is generously provided by Professor Zang Jianye, University of Science and Technology of China. The TEVp variant with five amino acid mutations are purified previously [23 (link)].

Zhou C., Yan Y., Fang J., Cheng B, & Fan J. (2014). A new fusion protein platform for quantitatively measuring activity of multiple proteases. Microbial Cell Factories, 13, 44.

+ Open protocol

+ Expand

Enzymatic and Chemical Quantitation of Formaldehyde

Check if the same lab product or an alternative is used in the 5 most similar protocols

Alcohol dehydrogenase from Saccharomyces cerevisiae (yeast) was purchased from Sigma-Aldrich as a lyophilized powder and used as received. ADH (~50 units) was mixed with 200 μM NADH and 400 μL of CYP121 reaction flow-through from the previous section. The absorbance was monitored at 339 nm using a UV–vis spectrophotometer (Agilent 8453) during the reaction.
Formaldehyde dehydrogenase from Pseudomonas was purchased from Sigma-Aldrich as a lyophilized powder and used as received. FDH (~0.5 units) was mixed with 200 μM NAD⁺ and CYP121 reaction flow-through, and the reaction was monitored as described above.
Chemical method of formaldehyde formation quantitation was also attempted. 2,4-Dinitrophenylhydrazine (2,4-DNP) was purchased from Sigma-Aldrich and prepared using previously published methods.²⁷ The reaction of CYP121 with cYF-4-OMe was completed as described above and heated to precipitate the enzyme, and then the solids were pelleted. The supernatant was collected, and 2, 4-DNP was added to make up 1.5% of the mixture. The resulting mixture was then analyzed via HPLC, utilizing a gradient of 5–95% acetonitrile in H₂O with 0.1% formic acid.

Nguyen R.C., Yang Y., Wang Y., Davis I, & Liu A. (2019). Substrate-Assisted Hydroxylation and O-Demethylation in the Peroxidase-like Cytochrome P450 Enzyme CYP121. ACS catalysis, 10(2), 1628-1639.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!