Iraffinity 1s spectrophotometer

The reagents and the solvents used for the synthesis and UV-vis studies were of analytical and spectroscopic grade. Phenyl hydrazine, hydrazine hydrate, ethyl 2-chloroacetate and ethyl acetoacetate were purchased from Spectrochem Pvt. Ltd and were used without further purification. All the amino acids used in UV-vis studies were purchased from Sigma Aldrich and Spectrochem Pvt Ltd. ¹H and ¹³C NMR were recorded at room temperature on a JNM-EXCP 400 (JEOL, USA) spectrometer using TMS as an internal standard and the chemical shifts are reported in parts per million (ppm). FTIR data was recorded using a Shimadzu IR Affinity 1S Spectrophotometer. The crude products were purified using column chromatography (silica gel 100–200 mesh) and methanol–chloroform as the solvent system. UV-vis experiments were performed on a Carey Series UV-vis spectrophotometer using a 1 cm quartz cuvette. All experiments were performed in compliance with the policy statement on ethical considerations involved in research on human subjects (ICMR, 1980). These experiments were approved by the departmental research committee of University of Delhi. The blood sample of a healthy volunteer was procured from a local pathology lab and arginine granule supplements were commercially available in the market. The donor provided informed consent in accordance with the guidelines of the institution.