Seedlings were cultivated as described above. To explore the effect of light intensity, seedlings were exposed to different light intensities (40 µmol m−2 s−1 or 296 µmol m−2 s−1 using confocal microscope equipped with a halogen lamp (HAL 100 W; Philips). The light intensity of confocal microscope halogen lamp (HAL 100 W; Philips) was determined using a Lutron LX-120 light meter (Lutron, Taipei, Taiwan) and converted into µmol m−2 s−1 using the formula as previously described68 . Each step took 30 s of illumination and imaging was immediately captured after each illumination period. After 3 min with 30 s intervals of exposure to the light, relaxation of seedlings in darkness was monitored for 4 min, during which the images were acquired every 30 s. Light intensity response curves for compartmentalized iNAP1 and iNAP4 were normalized by iNAPc.
Hal 100 w
Manufactured by Philips
Sourced in Taiwan, Province of China
The HAL 100 W is a laboratory equipment designed for high-accuracy weighing. It features a weighing capacity of up to 100 grams and a readability of 0.1 milligrams. The device is equipped with a digital display and provides precise measurements for various applications.
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Lab products found in correlation
3 protocols using hal 100 w
1
Light Intensity Effects on Compartmentalized iNAP Dynamics
2
Photosynthetic Light Response Dynamics
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Plants were exposed to 216 µmol m−2 s−1 white light from a halogen lamp (HAL 100 W; Philips) installed on the confocal microscope. The light intensity of the halogen lamp was determined using a Lutron LX-120 light meter (Lutron, Taipei, Taiwan). Each step took 30 s of irradiance and images were immediately captured after each irradiance period. After 3 min with 30 s intervals of white light exposure, images were acquired from seedlings in darkness every 30 s for 4 min. The results of iNAP4 sensor were normalized with stromal iNAPc.
3
Multimodal Confocal Imaging of Plant Cells
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Fluorescent images were obtained under a Zeiss LSM710/780 confocal microscope (Carl Zeiss Microscopy). Imaging was performed with a 40× and 63× oil-immersion lens in multitrack mode with line switching. cpYFP was excited at 488 (I488) and 405 nm (I405), and the emission signals were detected at 505–530 nm. AT1.03-nD/nA was excited at 458 nm at 7.5%, 10%, and 15% of maximal power for protoplasts, seedlings, and chloroplasts, respectively, and the emission signals were detected at 470–507 nm (Em470–507, mseCFP image) and 526–545 nm (Em526–545, FRET image). AT1.03-nD/nA was also excited at 515 nm (0.18% of maximal power for all samples) to directly excite Venus and detected at 526–545 nm (cp173Venus image). Chlorophyll fluorescence was detected at 629–700 nm. SYTOX orange nucleic acid stain was excited at 543 nm (1% of maximal power), and the emission signals were detected at 565–604 nm. White light illumination was achieved using a halogen lamp (HAL 100 W; Philips) attached to the confocal system.
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