Anti angptl1

Immunohistochemical analysis was performed using previously established method^{33 (link)}. Following primary antibodies were used: anti-VCAM-1, anti-ANGPTL1 and anti-PLAT (Abcam, UK). As secondary antibody anti-rabbit/mouse ImPRESS universal staining kit (Vector Laboratories, CA, USA) was applied. Sites of reactions were visualized as previously described^{34 (link)}. Intensity was calculated according to 3 points scoring system (0, no staining; 1, weak; 2, mild; 3, strong staining).

Western blots were performed as previously described [19 (link)]. Briefly, cells were lysed with a lysis buffer for 30 min on ice. Fifty micrograms of protein were fractionated via 10% SDS-PAGE and transferred onto nitrocellulose membranes (EMD Millipore, Billerica, MA, USA). The membranes were then probed with appropriate primary antibodies (1:500 dilution), including anti-AGO2, anti-VEGF, anti-ANGPTL1, anti-HIF-3α and anti-tubulin antibodies (Abcam, Cambridge, UK), followed by incubation with HRP-conjugated secondary antibodies (1:5000 dilution). The probed proteins were then detected with an enhanced chemiluminescent substrate (NEL100001EA; PerkinElmer). The tubulin expression level served as an internal control.