Vevo 3100 high resolution imaging system

Manufactured by Fujifilm

Sourced in Canada

The Vevo 3100 High-Resolution Imaging System is a lab equipment product designed for high-resolution imaging. It provides advanced capabilities for detailed visualization and analysis of biological samples.

Automatically generated - may contain errors

Lab products found in correlation

Vevolab software, by Fujifilm (3 mentions) Measurement software, by Fujifilm (1 mentions) Mx550d transducer, by Fujifilm (1 mentions) Vevostrain software, by Fujifilm (1 mentions) Vevo lab version 3, by Fujifilm (1 mentions) Isoflurane, by Baxter (1 mentions) C57bl 6j mice, by Jackson ImmunoResearch (1 mentions)

18 protocols using vevo 3100 high resolution imaging system

Echocardiographic Assessment of Cardiac Function

Check if the same lab product or an alternative is used in the 5 most similar protocols

Echocardiography was performed using Vevo 3100 High‐Resolution Imaging System (VisualSonics Inc, Toronto, Canada). Mice were sedated with 3% isoflurane and heart rate was maintained at 450–460 beats per minute by adjusting isoflurane concentration 1–1.5%. Short axis B‐ and M‐mode 2D echocardiograms were recorded through the anterior and posterior LV walls at the level of the papillary muscle. Left ventricular internal diameter at end‐systole (LVIDs) and end‐diastole (LVIDd), left ventricular posterior wall thickness, end‐systole (LVPWs) and end‐diastole (LVPWd), and fractional shortening (FS) and ejection fraction (EF) were measured by the VisualSonics Measurement Software.

Kumar A., Supowit S., Potts J.D, & DiPette D.J. (2019). Alpha‐calcitonin gene‐related peptide prevents pressure‐overload induced heart failure: role of apoptosis and oxidative stress. Physiological Reports, 7(21), e14269.

+ Open protocol

+ Expand

Echocardiographic Assessment of Cardiac Function in Mice

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

Noninvasive transthoracic echocardiography was used to evaluate left ventricular geometry and function in mice at baseline (3 days before TAC or sham TAC surgery), 6 weeks post TAC, and 8 weeks post COP using 1.5% isoflurane. Echocardiography was performed with a VisualSonics Vevo 3100 High-resolution Imaging System.¹⁷ (link)

Ge Z.D., Boyd R.M., Lantz C., Thorp E.B, & Forbess J.M. (2022). Cardio-omentopexy requires a cardioprotective innate immune response to promote myocardial angiogenesis in mice. JTCVS Open, 10, 222-242.

+ Open protocol

+ Expand

Echocardiographic Evaluation of Murine Cardiac Function

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

A Vevo 3100 High-Resolution Imaging System (VisualSonics Inc, Toronto, Canada) was used to perform the echocardiography in the mice (Kumar et al., 2019b (link)). Mice were sedated under 2% isoflurane and their heart rate was maintained at 450 ± 20 beats per minute. Short axis B- and M-mode 2D echocardiograms were recorded through the anterior and posterior LV walls at the level of the papillary muscle. Fractional shortening (FS) and ejection fraction (EF) were calculated by the VisualSonics Measurement Software.

Kumar A., Belhaj M., DiPette D.J, & Potts J.D. (2021). A Novel Alginate-Based Delivery System for the Prevention and Treatment of Pressure-Overload Induced Heart Failure. Frontiers in Pharmacology, 11, 602952.

+ Open protocol

+ Expand

Echocardiographic Assessment of Cardiac Function

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cardiac function was assessed by transthoracic echocardiography (TTE) using a Vevo 3100 high-resolution imaging system (VisualSonics) or iE33 Doppler ultrasonography system (Philips) in this study, under light anesthesia. The images were obtained with the subjects at rest and lying in the lateral decubitus position. The heart was imaged in the two-dimensional parasternal short-axis view, and an M-mode echocardiogram of the midventricle was recorded at the level of the papillary muscles. Diastolic-(LVIDd) and systolic-LV diameters (LVIDs) were measured. Fractional shortening and ejection fraction were calculated.

Xu Y., Zhang C., Jiang K., Yang X., Chen F., Cheng Z., Zhao J., Cheng J., Li X., Chen X., Zhou L., Duan H., Huang Y., Xiang Y, & Li J. (2022). Structural repurposing of SGLT2 inhibitor empagliflozin for strengthening anti-heart failure activity with lower glycosuria. Acta Pharmaceutica Sinica. B, 13(4), 1671-1685.

+ Open protocol

+ Expand

Echocardiographic Assessment of Cardiac Function

Check if the same lab product or an alternative is used in the 5 most similar protocols

To quantify left ventricle function, we performed echocardiograms on mice before and seven days after myocardial I/R injury. Mice were anesthetized with 2% isoflurane i.n. and placed on a heating pad. Chest hair was removed using an electric shaver and animals were fixated on their backs. Echocardiography loops were recorded in B and two-dimensional-targeted M modes in longitudinal and short-axis views on a Vevo 3100 High-Resolution Imaging System equipped with an MX550D transducer (VisualSonics, Toronto, ON, Canada). Mice were fixed on a heated table and heart rate was monitored during the procedure. Systole and diastole were defined based on concomitant electrocardiography (ECG) recordings. The end-systolic time point for left ventricle diameter measurement was defined as the maximum ventricle contraction just before the complete closure of the aortic valve. End-diastole was defined as the maximum left ventricle dilation and filling just before mitral valve closing (when visible) and aortic valve opening. Left ventricular ejection fraction was determined by left ventricle tracing relating the end-systolic left ventricle area as the minimal left ventricle cross-sectional area to the end-diastolic left ventricle area as the maximum left ventricle cross-sectional area in long-axis views. Fractional shortening was assessed by using VevoLab software (VisualSonics).

Liu W., Cronin C.G., Cao Z., Wang C., Ruan J., Pulikkot S., Hall A., Sun H., Groisman A., Chen Y., Vella A.T., Hu L., Liang B.T, & Fan Z. (2022). Nexinhib20 inhibits neutrophil adhesion and β2 integrin activation by antagonizing Rac-1-GTP interaction. Journal of immunology (Baltimore, Md. : 1950), 209(8), 1574-1585.

+ Open protocol

+ Expand

Minimally Invasive Transaortic Constriction in Mice

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

Minimally invasive TAC was performed on male C57BL/6 mice (n = 25; 13 mice in the TAC and 12 mice in the TAC+COP group) at 8 to 10 weeks of age, as described.¹² Using anesthesia of 1.5% to 2.0% isoflurane, a 0.5-cm horizontal incision was made at the level of the suprasternal notch. The chest was opened 2 to 3 cm in the proximal portion of the sternum. The thymus was deflected to expose the aortic arch. The transverse aorta was constricted between the innominate and left common carotid arteries using a 7-0 prolene suture ligature tied against a 25-gauge blunted needle (Figures E1 and E2). The latter was quickly removed to yield a constriction of 0.4 mm in diameter, which was measured using a VisualSonics Vevo 3100 High-resolution Imaging System.¹³ (link) Sham control mice (n = 24; 12 mice in the control group and 12 mice in the TAC group) underwent all surgical procedures without aortic constriction.

+ Open protocol

+ Expand

Echocardiographic Assessment of Murine RV

Check if the same lab product or an alternative is used in the 5 most similar protocols

Echocardiography was performed as previously described.¹¹, ¹², ²⁴, ²⁵ Mice were anesthetized with isoflurane and maintained a heart rate of 350 to 450 beats per minute and the Vevo 3100 High Resolution Imaging System (VisualSonics, Toronto, Canada) was used. RV thickness, RV end‐diastolic diameter, and tricuspid annular plane systolic excursion (TAPSE) were recorded in the M‐Mode. TAPSE was calculated by measuring the vertical movement of the tricuspid annulus between end‐diastole and end‐systole in the 4‐chamber view, reflecting the longitudinal contraction of the RV. Cardiac output (CO) was calculated after determining the pulmonary artery diameter, pulmonary artery velocity time integral, and heart rate using the formula: CO=7.85×PAD²×pulmonary artery velocity time integral×heart rate/10 000. Images with heart rate <350 were excluded from the analysis.

Zhang Y., Zervopoulos S.D., Boukouris A.E., Lorenzana‐Carrillo M.A., Saleme B., Webster L., Liu Y., Haromy A., Tabatabaei Dakhili S.A., Ussher J.R., Sutendra G, & Michelakis E.D. (2021). SNPs for Genes Encoding the Mitochondrial Proteins Sirtuin3 and Uncoupling Protein 2 Are Associated With Disease Severity, Type 2 Diabetes, and Outcomes in Patients With Pulmonary Arterial Hypertension and This Is Recapitulated in a New Mouse Model Lacking Both Genes. Journal of the American Heart Association: Cardiovascular and Cerebrovascular Disease, 10(23), e020451.

+ Open protocol

+ Expand

Echocardiographic Characterization of Mouse Cardiac Function

Check if the same lab product or an alternative is used in the 5 most similar protocols

Mice, minimally anesthetized with 1%–3% isoflurane, were studied by transthoracic echocardiography using a Vevo 3100 High-Resolution Imaging System (VisualSonics, Toronto, ON, Canada). For M-mode echocardiography, calculated parameters from at least three cardiac cycles were as follows: FS, fractional shortening = (LVID; d – LVID; s)/(LVID; d) and relative wall thickness = (LVAW; d + LWPW; d)/(LVID; d) in which LVID, LVAW, and LVPW are left ventricular interior diameter, anterior wall thickness, and posterior wall thickness, respectively, and d and s refer to diastole and systole, respectively. For B-mode echocardiography using both short and long axis parasternal imaging: LV Volume = 5/6∗(Endocardial Area)∗(Endocardial Major Axis); LV Wall Thickness = average wall thickness as calculated from the epicardial and endocardial area tracings; and ejection fraction = (Volume; d – Volume; s)/Volume; d. All parameters were measured or calculated using VevoLAB software (VisualSonics).

Li X., Li J., Samuelsson A.M., Thakur H, & Kapiloff M.S. (2024). Protein phosphatase 2A anchoring disruptor gene therapy for familial dilated cardiomyopathy. Molecular Therapy. Methods & Clinical Development, 32(2), 101233.

+ Open protocol

+ Expand

Echocardiographic Assessment of Cardiac Function in Mice

Check if the same lab product or an alternative is used in the 5 most similar protocols

Transthoracic echocardiography was performed before and 3, 7, 14 and 21 days after MI on mildly anesthetized spontaneously breathing mice (sedated by inhalation of 1% isoflurane, 1 L/min oxygen), using a Vevo 3100 High Resolution Imaging system (Visualsonics, Toronto, Canada). The mice were placed on a heated ECG platform and left parasternal long axis view and left mid-papillary short axis views were acquired in B-Mode and M-Mode, respectively.
End-diastolic volumes, end-systolic volumes, stroke volumes, ejection fractions and fractional shortening were evaluated on the left parasternal long axis view. The left ventricular anterior wall thickness was measured on the left mid-papillary short axis view in systole and diastole and the average between heart cycles was calculated. Vector displacement and 3-D wall displacement were generated and cardiac output was calculated using the Vevo software.

Schloss M.J., Horckmans M., Guillamat-Prats R., Hering D., Lauer E., Lenglet S., Weber C., Thomas A, & Steffens S. (2019). 2-Arachidonoylglycerol mobilizes myeloid cells and worsens heart function after acute myocardial infarction. Cardiovascular research, 115(3).

+ Open protocol

+ Expand

Echocardiography of Newborn Mice

Check if the same lab product or an alternative is used in the 5 most similar protocols

For echocardiography, newborn mice were imaged using the Vevo 3100 High Resolution Imaging System (FujiFilm VisualSonics Inc.), an ultra-high frequency linear array transducer (MX700). All the echocardiogram analyses were performed blinded (mice assigned to an alphanumeric code) until statistical analysis.

Gonzalez-Teran B., Pittman M., Felix F., Thomas R., Richmond-Buccola D., Hüttenhain R., Choudhary K., Moroni E., Costa M.W., Huang Y., Padmanabhan A., Alexanian M., Lee C.Y., Maven B.E., Samse-Knapp K., Morton S.U., McGregor M., Gifford C.A., Seidman J.G., Seidman C.E., Gelb B.D., Colombo G., Conklin B.R., Black B.L., Bruneau B.G., Krogan N.J., Pollard K.S, & Srivastava D. (2022). Transcription Factor Protein Interactomes Reveal Genetic Determinants in Heart Disease. Cell, 185(5), 794-814.e30.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!