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7 protocols using sodium citrate dihydrate

1

Synthesis of Europium-Doped Luminescent Materials

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All chemicals were of analytical grade and were used as received without further purification. H3PO4 (A.R.), HNO3 (A.R.), NH3·H2O (A.R.), La(NO3)3·6H2O (A.R.), sodium citrate dihydrate, Ce(NO3)3·6H2O (A.R.) and Eu2O3 (purity > 99.99%) were all supplied by Sinopharm Chemical Reagent Limited Corporation. The europium nitrate was prepared by dissolving Eu2O3 in 10% nitric acid, and then evaporated and dried in vacuum.
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2

Antimicrobial Hydrogel Synthesis and Evaluation

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Sodium citrate dihydrate, sodium chloride (NaCl), ferric chloride hexahydrate (FeCl3·6H2O), anionic polyacrylamide (PAM, Mn = 3 000 000), urea and polyethylene glycol-2000 (PEG, Mw = 2000), polyoxymethylene and hydrogen peroxide (H2O2) were purchased from Sinopharm Chemical Reagent Co., Ltd (China). Dimethyl sulfoxide (DMSO) and 3,3′,5,5′-tetramethylbenzidine (TMB) were purchased from Aladdin Reagent Co., Ltd (Shanghai, China). Fetal bovine serum and Dulbecco’s Modified Eagle Medium were obtained from Gibco (USA). Phosphate-buffered saline (PBS) was purchased from HyClone (USA), Cell Counting Kit (CCK-8) and Calcein-AM/propidium iodide (PI) staining kit was purchased from Shanghai Yeasen Biotechnology Co., Ltd (China). Beef extract, peptone and agar were all purchased from Shanghai Yuanye Biotechnology Co., Ltd (China). Escherichiacoli (CICC 10389) and S. aureus (CICC 21600) were obtained from the China Center of Industrial Culture Collection (CICC, Wuhan, China).
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3

Synthesis of Multifunctional Hydrogels

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Acrylamide (AM), β-cyclodextrin (β-CD), maleic anhydride (MAH), N,N-dimethylformamide (DMF), chloroform (CHCl3), acetone, 2-acrylamido-2-methyl-1-propanesulfonic acid (AMPS), ethylene glycol, N, N’-methylenebisAcrylamide (MBAA), ammonium persulfate (APS), N,N,N’,N’-tetramethylenediamine (TMEDA), cadmium chloride (CdCl2-2.5H2O), N-acetyl-L-cysteine (NAC), tellurium powder (Te) and sodium borohydride (NaBH4) were purchased from Sigma-Aldrich and used as received. Carbamide, zinc citrate dehydrate, sodium citrate dihydrate were purchased from Sinopharm Chemical Reagent Co. (Shanghai, China); Fe3O4 nanoparticles (20 nm) were purchased from Aladdin-reagent (Shanghai, China). Deionized water was used in all of the experiments.
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4

Synthesis of Metal-Organic Complexes

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The chemicals used in this study, including Zn(NO 3 ) 2 •6H 2 O, Fe(NO 3 ) 3 •9H 2 O, urea, glycerol, isopropanol, ethanol, HAuCl 4 •4H 2 O, and sodium citrate dihydrate (with a relative molar mass of 258.07 g/mol), were obtained from Sinopharm (Beijing, China). The reagents were of analytical grade and used without further purification.
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5

Synthesis and Characterization of Alginate-based Membranes

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Piperazine (PIP, 99%) and 1,3,5-benzenetricarbonyl trichloride (TMC, 98%) were purchased from Sigma-Aldrich, St. Louis, MO, USA. Sodium alginate (SA, AR) was obtained from Shanghai Makclin Biochemical Co., Ltd., Shanghai, China. Calcium sulfate anhydrous (CaSO4, CP), sodium sulfate (Na2SO4, 99%), sodium chloride (NaCl, 99.5%), magnesium sulfate (MgSO4, 98%), magnesium chloride (MgCl2, 98%), potassium chloride (KCl, 99.5%), sodium hydrate (NaOH, 96%), hydrochloric acid (HCl, 36–38%), sodium citrate dihydrate (99%), N,N-dimethylformamide (99.5%), dichloromethane (99.5%) and n-hexane (97%) were procured from Sinopharm Chemical Reagent Co. Ltd., Shanghai, China. Polyethersulfone microfiltration substrate (average pore size: 0.22 μm) was purchased from Haining Xindongfang Technology Co. Ltd., Haining, China. All the materials were used as received without further treatment. Ultrapure water (18.2 MΩ) used in all experiments was produced by an ELGA LabWater system (VWS Ltd., High Wycombe, UK).
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6

Procurement of Analytical Reagents for Chemical Analyses

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Nile red, Nile blue A, and sodium hydroxide were purchased from Sigma-Aldrich Co. (Missouri, United States); 1,2-propanediol, hydrochloric acid, ammonium thiocyanate were obtained from Merck KGaA (Darmtstadt, Germany); potassium dihydrogen phosphate, sodium borate, sodium borohydride, sodium citrate dihydrate, barium chloride, ferrous sulfate, and ferrous iron were purchased from Sinopharm chemical reagent Co., Ltd (Shanghai, China); isopropanol, acetonitrile, pentane, ethyl acetate, hexane, isooctane, methol, and butanol were purchased from Thermo Fisher Scientific (Waltham, USA). Furosine*HCl was purchased from Iris Biotech GmbH (Marktredwitz, Germany); Nε-carboxymethyl-l-lysine was purchased from Toronto Research Chemicals Inc. (Toronto, Canada); l-lysine was purchased from Anpel Laboratory Technologies Inc. (Shanghai, China); FMOC N-hydroxysuccinimide ester (≥98 %, chromatographic grade) and Cumene hydroperoxide (80 %, technical grade), propanal (97 %) and hexanal (98 %) were purchased from Sigma-Aldrich Co. (Missouri, United States); Propionaldehyde-2,2,3,3,3,-d5 (98.7 %, chromatographic grade) and hexanal-d12 (98.5 %, chromatographic grade) were purchased from CDN isotopes Inc. (Quebec, Canada).
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7

Titanium-Tin Oxide Nanocomposite Synthesis

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All chemicals were analytical-grade and used without further purification. Titanium butoxide (Ti(OC4H9)4), tin dichloride dehydrate (SnCl2·2H2O), sodium citrate dihydrate (Na3C6H5O7·2H2O, 99%), ethanol, and hydrochloric acid (HCl, 36.5–38% by weight) were purchased from Sinopharm Chemical Reagent Co., Ltd. Commercial carbon fiber papers were provided by FuelCellStore.
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