Cfpac 1

Three human pancreatic cancer cell lines, namely, PANC‑1, CFPAC-1, and MIAPaCa-2 were purchased from Shanghai Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences (Shanghai, China). PANC‑1 cells were cultured in Dulbecco's modified Eagle's medium (DMEM, Invitrogen) containing 10% fetal bovine serum (FBS, Gibco). CFPAC-1 cells were cultured in Iscove's Modified Dulbecco's Medium (IMEM, Invitrogen) containing 10% FBS (Gibco). MIAPaCa-2 cells were cultured in DMEM (Invitrogen) containing 10% FBS (Gibco), 2.5% Horse Serum (Gibco), and1% sodium pyruvate (Gibco). The normal human pancreatic cell line, HPDE6-C7, was purchased from Genio Biotechnology Co., Ltd. (Guangzhou, China). HPDE6-C7 cells were cultured in minimum essential medium (MEM, Invitrogen) supplemented with 10% FBS (Gibco). The cells were maintained in a humidified incubator at 37˚C in an atmosphere of 5% CO₂.

The human pancreatic cell lines, PANC-1, MIA PaCa-2, Capan-1, AsPC-1, CFPAC-1, and Hs766T, were obtained from the American Type Culture Collection (Manassas, VA, U.S.A) The PANC-1 and MIA PaCa-2 in Dulbecco’s Modified Eagle Medium (DMEM: Sigma-Aldrich, St Louis, MO, USA) with 10% fetal bovine serum (FBS) and antibiotics (1% penicillin and streptomycin), CFPAC-1 and Capan-2 in Iscove’s Modified Dulbecco’s Medium (IMDM: Thermo Fisher Scientific, Waltham, MA, USA) with 10% FBS and antibiotics, and AsPC-1 cells in RPMI-1640 medium (Thermo Fisher Scientific) with 10% FBS and antibiotics. Mouse primary pancreatic cells were cultured and maintained as described previously [26 (link)]. Murine PDAC (KPC1) and paired metastases (KPC1Liv) cell lines were provided by Dr. Sunil Hingorani (University of Washington). In brief, KPC1 cell lines were established from primary PDAC of a genetically engineered mouse model of PDAC (LSL-Kras^G12D/+;p53^R172H/+;Pdx1-cre) [32 (link)], whereas the KPC1Liv cell lines were isolated from paired liver metastases arising in KPC1 mice. KPCY cells are derived from a Pdx1-cre;LSL-Kras^{G12D/+;p53fl/+};R26^YFP mouse (KPCY mice) and were provided by Dr. Andrew D. Rhim (The University of Texas MD Anderson Cancer Center).

Human pancreatic cancer cell lines Capan-1 (adenocarcinoma, liver metastasis) and PANC-1 (epithelioid carcinoma) were purchased from Korean Cell Line Bank (KCLB, Seoul, Korea). KCLB characterized the cell lines using DNA fingerprinting analysis, species verification test, mycoplasma contamination test and viral contamination test. Human pancreatic cancer cell lines, Capan-2 (adenocarcinoma) and CFPAC-1 (adenocarcinoma, liver metastasis), were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA). The cell lines were characterized by the tests for morphology, post-freeze viability, interspecies determination (isoenzyme analysis), cytogenetic analysis, mycoplasma contamination and bacterial and fungal contamination. Capan-1 cells were cultured with RPMI-1640 medium (Thermo Fisher Scientific, Waltham, MA, USA), Capan-2 cells in McCoy’s 5A medium (Thermo Fisher Scientific), PANC-1 cells in Dulbecco’s modified Eagle’s medium (DMEM, Thermo Fisher Scientific) and CFPAC-1 cells in Iscove’s modified Dulbecco’s medium (IMDM, Thermo Fisher Scientific). All cell lines were cultured with 10% fetal bovine serum (FBS, Thermo Fisher Scientific), 100 U/mL penicillin and 100 µg/mL streptomycin and were incubated at 37 °C in 5% CO₂.

Human pancreatic cancer cell lines Panc-1 and CFPAC-1 were obtained from the Committee of Type Culture Collection of Chinese Academy of Sciences (Shanghai, China). Panc-1 cells were cultured in Dulbecco's modified Eagle medium (DMEM) (Thermo Fisher Scientific, USA) medium with 10% fetal bovine serum and 1% penicillin-streptomycin solution (100 U/ml) in the incubator containing 5% CO2 at 37 °C. CFPAC-1 cells were cultured in IMDM (Thermo Fisher Scientific, USA) medium with 10% fetal bovine serum and 1% penicillin-streptomycin solution (100 U/ml) in the incubator containing 5% CO2 at 37 °C. Panc-1 SOX8 expressing cells were also cultured in DMEM and CFPAC-1 SOX8 knock-down cells were also cultured in IMDM.

Four pancreatic cancer cell lines (PANC-1, BXPC-3, ASPC-1 and CFPAC-1) were purchased from the Cell Bank of the Chinese Academy of Sciences (Shanghai, China). PANC-1 and BXPC-3 were cultured in Dulbecco’s modified Eagle medium (Thermo Fisher Scientific, South America) containing 10% fetal bovine serum (Thermo Fisher Scientific). ASPC-1 was cultured in RPMI-1640 (Thermo Fisher Scientific) containing 10% fetal bovine serum (Thermo Fisher Scientific). CFPAC-1 was cultured in Dulbecco’s modified Eagle medium (Thermo Fisher Scientific) containing 10% fetal bovine serum (Thermo Fisher Scientific). All cell lines were cultured at 37 °C under 5% CO₂/95% air and revived every 3 to 4 months. HSF1 and second mitochondria-derived activator of caspase (SMAC)-specific siRNAs were prepared and used in transfections as described previously [15 (link)].

The human pancreatic cancer cell lines CFPAC-1 and PL45 were purchased from the Chinese Academy of Science Cell Bank. The human pancreatic cancer cell lines Capan-1 and PANC-1 were a gift from Dr. Yuan Chen (City of Hope). The human erythroleukemic cell line K562 was purchased from the American Type Culture Collection (ATCC). Capan-1 and CFPAC-1 cells were cultured in IMDM with 20% fetal bovine serum (FBS) and 1% antibiotic (Thermo Fisher Scientific, USA). MiaPaCa-2, PL45, and PANC-1 cells were cultured in DMEM with 10% FBS and 1% antibiotic. K562 cells were cultured in RPMI 1640 medium supplemented with 10% FBS and 1% antibiotic. All the cell lines were incubated under standard culture conditions (37°C and 5% CO₂). PRMI 1640, DMEM, IMDM, and FBS were purchased from Gibco (Cambrex, MD, USA).